Browsing by Author "Martingo, Maria"
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- An uplifting avenue upon mealworm chitosan for hemodialysis applicationPublication . Martingo, Maria; Baptista-Silva, Sara; Mesquita, Raquel; Ferreira, João Paulo; Borges, Sandra; Pintado, ManuelaChronic kidney disease (CKD) is characterized by a continuous decline in kidney function, often persisting for years or even a lifetime. This study presents a new exploration into the extraction and application of insect chitosan (Tenebrio molitor) targeting hemodialysis (HD). Initially, the potential of insect-derived chitosan as a sustainable and effective alternative to traditional sources (i.e. crustaceans) is demonstrated. Efficient extraction methods were developed (totaling between 6 h and 12 h), producing chitosan. Firstly, chitin was efficiently isolated from T. molitor through deproteinization and demineralization, with yields of approximately 5% (w/w). Subsequently, chitosan was obtained from the extracted chitin resulting in yields of between 65.0 ± 0.8% (w/w) and 79.3 ± 0.8% (w/w). Characterization using Fourier Transform Infrared Spectroscopy (FTIR) confirmed structural similarities with commercial chitosan and degrees of deacetylation in the 73 - 75% range. The bioactive properties of chitosan obtained from T. molitor, including antimicrobial and antioxidant activities, were evaluated. All the microorganisms tested were inhibited, exhibiting minimum lethal concentrations between 2 and 8 mg/mL, confirming the antimicrobial activity of the extracted chitosan. In addition, chitosan showed antioxidant activity in the range of 60 to 65 µmol Trolox equivalent/g, suggesting its viability for various medical applications. This study also developed a chitosan-based membrane (CH - M) culminating in a proof-of-concept phase for hemodialysis. The permeation characteristics of CH-M for urea and albumin were studied in vitro to assess their suitability as HD membranes. Urea was permeable to values of over 70% and albumin was retained.
- Exploring the potential of mealworm chitosan for hemodialysis applicationsPublication . Martingo, Maria; Baptista-Silva, Sara; Mesquita, Raquel; Ferreira, João Paulo; Borges, Sandra; Pintado, ManuelaThis study introduces a sustainable and efficient alternative to traditional chitosan sources derived from crustaceans, exploring the extraction and application of insect-derived chitosan from Tenebrio molitor for hemodialysis (HD) membranes design. Efficient extraction and deacetylation methods were tested and developed between 6 h and 12 h to obtain chitosan. Chitin was isolated from T. molitor through deproteinization and demineralization, with yields of approximately 5 % (w/w). Chitosan was obtained from the extracted chitin resulting in yields of between 65.0 and 79.3 (w/w). Characterization using FTIR confirmed structural similarities with commercial chitosan and degrees of deacetylation in the 73–75 % range. The bioactive properties of chitosan obtained from T. molitor, including antimicrobial and antioxidant activities, were evaluated. All the microorganisms tested were inhibited, exhibiting minimum lethal concentrations between 2 and 8 mg/mL. In addition, chitosan showed antioxidant activity in the range of 60–65 μmol Trolox equivalent/g, suggesting its viability for various medical applications. This study additionally allowed the design of sustainable hybrid chitosan membrane (CH-M) tailored for HD applications. The permeation characteristics of CH-M for urea and albumin were studied in vitro to assess their suitability as HD membranes. Urea was permeable to values of over 70 % and albumin was retained. Also, cytotoxicity assays against L929 fibroblast cells demonstrated that the CH-M samples exhibit low metabolic inhibition (around 15 %) The application of CH-M in HD represents a significant advance, offering the potential for enhanced therapeutic outcomes for chronic kidney disease (CKD).