Browsing by Author "Fialho, Ana Cristina"
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- Effect of cerium-containing hydroxyapatite in bone repair in female rats with osteoporosis induced by ovariectomyPublication . Vieira, Ewerton; Silva, Marcos; Maia-Filho, Antonio; Ferreira, Daniel; Figuerêdo-Silva, José; Rovaris, Karla; Fialho, Ana Cristina; Leite-Oliveira, Ana; Oliveira, André L. Menezes de; Fonseca, Maria Gardênnia da; Osajima, Josy A.; Silva-Filho, Edon C. daOsteoporosis is a public health problem, with bone loss being the main consequence. Hydroxyapatite (HA) has been largely used as a bioceramic to stimulate bone growth. In our work, a cerium-containing HA (Ce-HA) has been proposed and its effects on the antimicrobial and bone-inducing properties were investigated. The synthesis of the materials occurred by the suspension–precipitation method (SPM). The XRD (X-ray Diffraction) confirmed the crystalline phase, and the Rietveld refinement confirmed the crystallization of HA and Ce-HA in a hexagonal crystal structure in agreement with ICSD n◦ 26205. Characterizations by FT-IR (Fourier Transform Infrared Spectroscopy), XPS (X-ray Photoemission Spectroscopy), and FESEM-EDS (Field Emission Scanning Electron Microscope-Energy Dispersive X-ray Spectroscopy) confirmed the presence of cerium (Ce3+ and Ce4+ ). The antibacterial activity of Has was evaluated against Staphylococcus aureus 25,923 and Escherichia coli 25,922 strains, which revealed that the material has antimicrobial properties and the cytotoxicity assay indicated that Ce-containing HA was classified as non-toxic. The effects of Ce-HA on bone repair, after application in bone defects in the tibia of female rats with osteoporosis induced by ovariectomy (OVX), were evaluated. After 15 and 30 days of implantation, the samples were analyzed by Raman, histology and X-ray microtomography. The results showed that the animals that had the induced bone defects filled with the Ce-HA materials had more expressive bone neoformation than the control group.
- Improving the identification of high-risk non-metastatic castration-resistant prostate cancer patients in clinical practicePublication . Rosinha, Alina; Rabaça, Carlos; Calais, Fernando; Pinto, João Moreira; Barreira, João Vasco; Fernandes, Ricardo; Ramos, Rodrigo; Fialho, Ana Cristina; Palma dos Reis, JoséNon-metastatic castration-resistant prostate cancer (nmCRPC) represents a challenging disease state in prostate cancer care. nmCRPC patients with a high risk of progression to metastatic disease who are identified by a prostate-specific antigen doubling time (PSADT) ≤10 months are eligible for treatment with the novel androgen receptor inhibitors (ARIs), shown to delay disease progression and extend survival. However, nmCRPC is often unexploited in clinical practice due to a lack of standardization in the methodology and in the tools used for its identification. In this article, a group of Urology and Oncology specialists with acknowledged expertise in prostate cancer reviews the state of the art in the management of high-risk nmCRPC patients, identifies gaps and unmet needs, and proposes strategies to optimize the identification of this patient subgroup in the clinical practice and improve their health outcomes.
- Scaffold based on castor oil as an osteoconductive matrix in bone repair: biocompatibility analysisPublication . Lima, Fabianne Soares; Matos, Luis Felipe; Pacheco, Isnayra Kerolaynne; Reis, Fernando; Câmara, João Victor Frazão; Pierote, Josué Junior Araujo; Matos, José Milton; Ribeiro, Alessandra; Moura, Walter; Fialho, Ana CristinaTo analyze the biocompatibility of the scaffold produced from a natural polymer derived from castor oil through hemolytic activity and antimicrobial activity, to enable the clinical application. Three in vitro tests were performed: Hemolytic activity test - Polymer partially dissolved in contact with blood agar; Hemolytic activity test in sheep's blood - Polymer extract with red blood cells solution; Antimicrobial activity test - Solid polymer in direct contact with E. Coli and S. Aureus. For hemolytic tests, none of the samples showed hemolysis. Negative hemolytic activity is a good indicator, as the maintenance of the blood clot in the area of the lesion is essential for the formation of new tissue. For the antimicrobial activity test, no significant activity was observed against the bacteria used. The polymer is not toxic to red blood cells, being viable for clinical application as a matrix for tissue regeneration.