Browsing by Author "Ferreira, Mariana"
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- ATG9A regulates the dissociation of recycling endosomes from microtubules to form liquid influenza A virus inclusionsPublication . Vale-Costa, Sílvia; Etibor, Temitope Akhigbe; Brás, Daniela; Sousa, Ana Laura; Ferreira, Mariana; Martins, Gabriel G.; Mello, Victor Hugo; Amorim, Maria JoãoAU It is:now Pleaseconfirmthatallheadinglevelsarerepresentedcorrectly established that many viruses that threaten public health : establish condensates via phase transitions to complete their lifecycles, and knowledge on such processes may offer new strategies for antiviral therapy. In the case of influenza A virus (IAV), liquid condensates known as viral inclusions, concentrate the 8 distinct viral ribonucleoproteins (vRNPs) that form IAV genome and are viewed as sites dedicated to the assembly of the 8-partite genomic complex. Despite not being delimited by host membranes, IAV liquid inclusions accumulate host membranes inside as a result of vRNP binding to the recycling endocytic marker Rab11a, a driver of the biogenesis of these structures. We lack molecular understanding on how Rab11a-recycling endosomes condensate specifically near the endoplasmic reticulum (ER) exit sites upon IAV infection. We show here that liquid viral inclusions interact with the ER to fuse, divide, and slide. We uncover that, contrary to previous indications, the reported reduction in recycling endocytic activity is a regulated process rather than a competition for cellular resources involving a novel role for the host factor ATG9A. In infection, ATG9A mediates the removal of Rab11a-recycling endosomes carrying vRNPs from microtubules. We observe that the recycling endocytic usage of microtubules is rescued when ATG9A is depleted, which prevents condensation of Rab11a endosomes near the ER. The failure to produce viral inclusions accumulates vRNPs in the cytosol andAU reduces: Pleasecheckandconfirmthattheeditst genome assembly and the release of infectious virions. We propose that the ER supports the dynamics of liquid IAV inclusions, with ATG9A facilitating their formation. This work advances our understanding on how epidemic and pandemic influenza genomes are formed. It also reveals the plasticity of recycling pathway endosomes to undergo condensation in response to infection, disclosing new roles for ATG9A beyond its classical involvement in autophagy.
- Avaliação do impacto do programa Nutrium Corporate Wellness na literacia nutricional em meio laboralPublication . Ferreira, Mariana; Pinto, Elisabete Cristina Bastos; Alves, José Carlos FigueiraO presente relatório de estágio tem como objetivo descrever todas as atividades realizadas no período de estágio, no âmbito da unidade curricular Dissertação de Mestrado para obtenção do grau de Mestre em Biotecnologia e Inovação, pela Escola Superior de Biotecnologia, da Universidade Católica Portuguesa. O meu estágio curricular foi desenvolvido na empresa Nutrium, onde tive a oportunidade de integrar a equipa de marketing. Ao longo do período de estágio foram desenvolvidas atividades que me permitiram aumentar os conhecimentos nas áreas de marketing digital e nutrição. Trabalhar e aprender sobre uma área tão distante da área de formação base, como é o marketing digital, permitiu-me expandir as minhas competências e crescer de forma pessoal e profissional. Foi um privilégio contribuir com o meu conhecimento em nutrição para o sucesso da equipa e fazer parte de uma mudança tão importante na empresa. Sinto-me privilegiada pelos meses que passei na empresa e estou confiante que a Nutrium vai ter um impacto imenso na saúde dos colaboradores pelo mundo. De forma geral, os objetivos do estágio foram cumpridos e este período foi imprescindível ao meu crescimento, como nutricionista e como pessoa.
- Do clear aligners release toxic chemicals? - a systematic reviewPublication . Ferreira, Mariana; Costa, Hélder; Veiga, Nélio; Correia, Maria J.; Gomes, Ana T. P. C.; Lopes, Pedro C.The use of advanced polymer materials in clear aligners, such as polyurethane and PET-G, has gained widespread acceptance due to their mechanical properties, biocompatibility and aesthetic appeal. However, concerns persist regarding their potential to release chemical compounds. Our goal is to understand the impact of the toxicity of clear aligners on oral cavity of orthodontic patients. An extensive systematic search was conducted in electronic databases PubMed, Scopus and Cochrane, to identify articles with relevant data. This systematic review will be conducted following the Preferred Reporting Items for Systematic reviews and Meta-analysis guidelines to answer a question formulated according to the Population, Intervention, Com-parison and Outcomes strategy. Four hundred and thirteen articles potentially relevant were identified and after applying PRISMA guidelines 7 articles were included in this review. Our results suggest that clear aligners are generally safe, concerns remain regarding the chemical leaching of thermoplastic materials, bacterial accumulation due to reduced saliva flow, and mild inflammatory responses. Our review empha-sizes that although most materials are biocompatible, some exhibit moderate cytotoxicity, underscoring the need for continued research and improvements in material composition.
- Do clear aligners release toxic chemicals? - a systematic reviewPublication . Ferreira, Mariana; Costa, Hélder; Veiga, Nélio; Correia, Maria J.; Gomes, Ana T. P. C.; Lopes, Pedro C.Clear aligners are a modern orthodontic solution designed to address dental malocclusions discreetly and effectively. In terms of clinical side effects, the most reported issues among aligner users are mild irritation of the oral mucosa, localized inflammation, and hypersensitivity. The use of advanced polymer materials in clear aligners, such as polyurethane and PET-G, has gained widespread acceptance due to their mechanical properties, biocompatibility, and aesthetic appeal. However, concerns persist regarding their potential to release chemical compounds. Our goal is to understand the impact of clear aligner toxicity on the oral cavity of orthodontic patients. An extensive systematic search was conducted in the electronic databases PubMed, Scopus, and Cochrane to identify articles with relevant data. This systematic review was conducted following the Preferred Reporting Items for Systematic reviews and Meta-analysis guidelines (PRISMA) to answer a question formulated according to the Population, Intervention, Comparison, and Outcomes. Four hundred and thirteen articles potentially relevant were identified and after applying PRISMA guidelines and inclusion/exclusion criteria, seven articles were included in this review. Our results suggest that clear aligners are generally safe, but concerns remain regarding the chemical leaching of thermoplastic materials, bacterial accumulation due to reduced saliva flow, and mild inflammatory responses. Our review emphasizes that although most materials are biocompatible, some exhibit moderate cytotoxicity, with the potential to impact these patients’ oral health, underscoring the need for continued research and improvements in material composition.