Browsing by Author "Fernandes, Gustavo"
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- Autologous Platelet Concentrate of 2 nd and 3 rd generations efficacy in the surgical treatment of gingival recession: an overview of systematic reviewsPublication . Fernandes, Gustavo; Santos, Nuno; Siqueira, Rafael; Wang, How Lay; Blanco-Carrion, Juan; Fernandes, JulianaBackground: Autologous platelet concentrate (APC)/platelet-rich fibrin (PRF) of second and third generations has increased use in periodontics to optimize wound healing. Few systematic reviews (SRs) have reported improved clinical outcomes, while other studies reported significantly better results for the connective tissue graft (CTG). There is still unclear clinical evidence about APC/PRF use to treat gingival recession (GR) defects. Then, the purpose of this SR was to evaluate the use of APC/PRF membranes (2 nd and 3 rd generations) in root coverage (RC) procedures and assess its efficacy as a substitute biomaterial. Materials and Methods: An electronic search was conducted in PubMed, Cochrane Central, Web of Science, Google Scholar, BookSC databases, and gray literature. The search strategy, without date restriction up to April 2020, included keywords as 'platelet-rich fibrin,' 'autologous platelet concentrates,' 'blood,' 'systematic review,' 'periodontics,' 'surgery,' 'tissue,' 'gingiva,' 'gingival recession,' 'connective tissue,' 'graft,' and 'root coverage.' The methodological quality was evaluated through the AMSTAR2, and a population, index test, comparator, outcome strategy was used to assess specific clinical parameters such as recession depth, clinical attachment levels, and RC outcomes. Results: Nine SRs were included. Only three articles described the technique of APC/PRF production. Three studies reported unfavorable outcomes using APC, while six reported favorable results and postoperative discomfort reduction. Articles included in this SRs that provided information about APC/PRF membranes (n = 13) showed no significant difference between APC/PRF and the control group for the parameters analyzed. Conclusions: This implies that APC/PRF may be considered a feasible substitute biomaterial for treating GR defects, although the CTG still provides superior outcomes. Further long-term and controlled studies are needed to verify this finding.
- De‐epithelialized connective tissue graft and the reminiscent epithelial content after harvested by the Harris’ technique: a histological and morphometrical case seriesPublication . Maia, Vinícius Tadeu Gonçalves; Kahn, Sérgio; Souza, Alex Balduino de; Fernandes, GustavoIntroduction: The remaining epithelial layer existent in connective tissue graft (CTG) harvested from the hard palate, which underwent de‐epithelization outside the oral cavity, can be histologically detected unless it is completely removed. Its presence may cause adverse esthetics results, affecting thus the color and texture of the receptor site, and an increased risk of presence of scar tissues after surgical procedures. The proposal of this study was to evaluate the CTG (histological and morphometrically) collected from the hard palate using the Harris’ technique, removing the epithelial layer outside the mouth, assessing the remaining presence of epithelial tissue. Case Series: Fourteen patients (14CTGs) were included in the present case series study, therefore there was two dropouts. A small part of the tip of the graft was harvested and fixed in formalin solution for histological processing, staining, and then to be morphometrically analyzed. The epithelial tissue and CTGs were assessed by three calibrated and double‐blinded professionals. All information was compiled and performed the statistical analysis. CTGs obtained had a width average of 1224.26μm. There was no presence of any residual of the epithelium in three samples, whereas only one had the epithelium tissue covering the entire connective layer. Furthermore, seven samples (approximately 50%) had the presence of epithelium. Conclusion: Within the limitation of this study, there was incomplete removal of the epithelial layer after harvesting the CTG using the Harris’ technique (44.32%), most likely due to its histological persistency, suggesting to be inaccurate the clinical removal.
- EditorialPublication . Fernandes, Gustavo
- A influência dos bifosfonatos na falha dos implantes dentários: revisão sistemáticaPublication . Rebelo, Cristiana; Fernandes, Gustavo; Malta, Nuno; Couto, Patrícia
- A protein profiling strategy for periodontal disease applications: the Perio-SalivaPRINTPublication . Rosa, Nuno; Esteves, Eduardo; Esteves, Ana Cristina; Fernandes, Gustavo; Correia, Maria; Siqueira, Walter L.; Barros, MarleneObjectives: It is known that several clinical situations have characteristic molecular deregulations. Some molecular data underlying these deregulations can be found in saliva and have been annotated in databases (SalivaTecDB). Strategies are needed to identify the phenotypes characteristic of these deregulations. Our group has developed a strategy that allows the establishment of saliva protein profiles reflecting different conditions (health and disease). These profiles can be integrated to clinical data (SalivaPRINT Toolkit). The present work aims to identify the Periodontal Diseases (PD)-specific protein profiles. Methods: Unstimulated whole saliva was collected from a group of healthy subjects and a group of PD patients (with gingivitis, periodontitis or periimplantitis). Salivary proteins were separated by the Experion™ automated capillary electrophoresis. The protein profiles of each condition were integrated with the corresponding protein data retrieved from our in-house database (SalivaTecDB). Results: The strategy used enabled the determination of a total protein profile from saliva characteristic of each PDs -the Perio-SalivaPrint. The use of the SalivaPrint Toolkit allowed the identification of molecular weight ranges altered in PD. Using SalivaTecDB we were able to suggest proteins potentially involved in the underlying dysregulated mechanisms of the disease. Conclusions: This approach enabled the determination of a Perio-SalivaPrint – protein profiles specific for gingivitis, periodontitis or periimplantitis - that could empower the use of saliva as a simple and less expensive diagnostic and monitoring fluid. The strategy presented could be an important tool for future applications in the early diagnostic/ screening of Periodontal Disease patients with applications in chairside monitoring.