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Percorrer por autor "Domingues, Pedro"

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  • Bioactive lipids in Dunaliella salina: implications for functional foods and health
    Publication . Pais, Rita; Conde, Tiago; Neves, Bruna B.; Pinho, Marisa; Coelho, Marta; Pereira, Hugo; Rodrigues, Alexandre M. C.; Domingues, Pedro; Gomes, Ana Maria; Urbatzka, Ralph; Domingues, Rosário; Melo, Tânia
    Dunaliella salina is a green microalga extensively explored for β-carotene production, while knowledge of its lipid composition is still limited and poorly investigated. Among lipids, polar lipids have been highlighted as bioactive phytochemicals with health-promoting properties. This research aimed to provide an in-depth lipidome profiling of D. salina using liquid and gas chromatography coupled with mass spectrometry. The lipid content was 6.8%, including phospholipids, glycolipids, betaine lipids, sphingolipids, triglycerides, diglycerides, and pigments. Among the total esterified fatty acids, 13.6% were 18:3 omega-3 and 14.7% were 18:1 omega-9. The lipid extract of D. salina showed anti-inflammatory activity by inhibiting cyclooxygenase-2 activity at 100 µg/mL, dose-dependent antioxidant scavenging activity, and antidiabetic activity by inhibiting α-glucosidase activity at 25 and 125 µg/mL. In conclusion, the lipid extract of D. salina has the potential to be used as a functional food ingredient or in the nutraceutical and cosmeceutical industries.
    2024-10-19Artigo científico Acesso aberto Ver mais
  • Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels
    Publication . Silva, Joana; Carvalho, Ana Sofia; Votorino, Rui; Domingues, Pedro; Teixeira, Paula; Gibbs, paul
    Protein extraction and the proteome of Lactobacillus delbrueckii subsp. bulgaricus were studied using different stains. The reversible silver staining technique was shown to be more sensitive than the irreversible silver stain. Coomassie colloidal was demonstrated to be as sensitive as reversible silver stain; however, the Coomassie colloidal blue solution developed a higher background and for sample preparation was more time-consuming.
    2004Artigo científico Acesso aberto Ver mais