Browsing by Author "Crespo, Rute"
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- Effect of a soil biofertilizer in the functional profile of the soil microbial community through the biolog assayPublication . Lima, Sonny; Pacheco, João; Ganilho, Catarina; Crespo, Rute; Andreani, Tatiana; Godinho, Mariana; Pereira, Sofia; Pereira, RuthBackground & Aim: The dependency on crops of products with chemical compounds, whether fertilizers or pesticides, for saving the development and yield has been a challenge to the environment, and new alternatives are being sought to change this reality. The excessive use of chemicals affects human health and soil quality. There is a significant need to produce green compounds that are environmentally sustainable, improving or restoring microbial activity in the soil. Biofertilizers are compounds that contain biological substances and may be capable of stimulating plant development, by providing nutrients and other plant-growth promoting compounds and by increasing soil microbial activity, and reducing the impacts generated by mineral fertilizers. The objective was to verify the effects of applying a biofertilizer, Vitasoil™, which contains rhizospheric microorganisms that positively interact with plants, on the functional profile of the soil microbial community. Methods: The first evaluation was conducted one month after the application of the compound, using Biolog EcoPlates. Results: Its composition includes two types of bacteria: Azotobacter sp. and Azospirillum sp. We chose kale (Brassica oleracea) for the tests, with direct application of the compound diluted in the proper proportion to the plant's root, so that we could compare the inoculated soils with the non- inoculated ones. Conclusions: This method was able to show the effects of the inoculant application on the soil with and without B. oleracea plants and inferring about the positive/negative effects on the non-target bulk soil microbial community.
- Effect of a soil biofertilizer in the functional profile of the soil microbial community through the biolog assayPublication . Lima-Filho, Sonny; Pacheco, João; Ganilho, Catarina; Crespo, Rute; Andreani, Tatiana; Godinho, Mariana; Pereira, Sofia; Pereira, RuthIntroduction: The dependency on crops of products with synthetic chemical compounds, whether fertilizers or pesticides, for saving the development and yield has been a challenge to the environment, and new alternatives are being sought to change this reality. The excessive use of chemicals affects human health and soil quality. Therefore, there is a significant need to produce green compounds that are environmentally sustainable, aimed at improving or restoring microbial activity in the soil. Biofertilizers are compounds that contain biological substances capable of stimulating plant development, by providing nutrients and other plant-growth promoting compounds, of increasing soil microbial activity, and of reducing the environmental impacts of mineral fertilizers. Aims: To assess the effects of applying a biofertilizer inoculum, containing rhizospheric microorganisms, that positively interact with plant roots, on the functional profile of the bulk soil microbial community; To evaluate soil microbial activity after 30 and 60 days of organic biofertilizer application through the BIOLOG assay. Conclusion: The soil without inoculum was the one with the lowest microbial activity (measured by the AWCD) after both incubation periods, although the differences do not seem to be significant (data under analysis); The presence of the plant was able to compensate for the possible effect of the biofertilizer on the highest consumption of some substrates in detriment of others, as suggested by the Eveness index (C, D); Both the plant and the biofertilizer inoculum promoted a higher soil functional biodiversity of the soil, as measured by the Shannon Index (E, F) and the Richness (G, H), calculated based on the number and type of carbon substrates mineralized by the soil microbial community.