Browsing by Author "Barbosa, Joana Cristina"
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- Achieving efficient viability of akkermansia muciniphila during aerobic storage and gastrointestinal passage through calcium-alginate encapsulationPublication . Machado, Daniela; Fonseca, Mariana; Vedor, Rita; Barbosa, Joana Cristina; Gomes, Ana Maria
- Aggregation properties of probiotic strains under aerobic and anaerobic conditionsPublication . Machado, Daniela; Andrade, José Carlos; Barbosa, Joana Cristina; Costa, Francisca; Fonseca, Mariana; Almeida, Diana; Freitas, Ana Cristina; Gomes, Ana Maria
- Aggregation properties of probiotic strains under aerobic and anaerobic conditionsPublication . Machado, Daniela; Andrade, José Carlos; Barbosa, Joana Cristina; Costa, Francisca; Fonseca, Mariana; Almeida, Diana; Freitas, Ana Cristina; Gomes, Ana Maria
- Akkermansia muciniphila antimicrobial susceptibility profilePublication . Barbosa, Joana Cristina; Machado, Daniela; Almeida, Diana; Andrade, José Carlos; Freitas, Ana Cristina; Gomes, Ana Maria
- Akkermansia muciniphila encapsulated in calcium-alginate hydrogelated matrix: viability and stability over aerobic storage and simulated gastrointestinal conditionsPublication . Machado, Daniela; Fonseca, Mariana; Vedor, Rita; Sousa, Sérgio; Barbosa, Joana Cristina; Gomes, Ana MariaAkkermansia muciniphila is considered a next-generation probiotic to be incorporated in new food and pharmaceutical formulations. Effective delivery systems are required to ensure high probiotic viability and stability during product manufacture, shelf-life, and post-consumption, namely, throughout digestion. Hydrogelated matrices have demonstrated promising potential in this dominion. Hence, this work aimed to evaluate the effect of a calcium-alginate hydrogelated matrix on A. muciniphila viability during 28-days refrigerated aerobic storage and when exposed to simulated gastrointestinal conditions, in comparison with that of free cells. Akkermansia muciniphila was successfully encapsulated in the calcium-alginate matrix via extrusion (60% encapsulation yield). Furthermore, encapsulated A. muciniphila exhibited high stability (a loss in viability lower than 0.2 log-cycle) after 28-days of refrigerated aerobic storage, maintaining its viability around 108 CFU/g. Prominently, as the storage time increased, encapsulated A. muciniphila revealed higher viability and stability regarding in vitro gastrointestinal conditions than free cells. This suggests that this encapsulation method may attenuate the detrimental effects of prolonged aerobic storage with a subsequent gastrointestinal passage. In conclusion, encapsulation via extrusion using a calciumalginate hydrogelated matrix seems to be a promising and adequate strategy for safeguarding A. muciniphila from adverse conditions encountered during refrigerated aerobic storage and when exposed to the gastrointestinal passage.
- Dark chocolate as a promising carrier for probiotic strainsPublication . Barbosa, Joana Cristina; Gomes, Ana Maria; Machado, Daniela; Silva, Mariana; Almeida, Diana; Andrade, José Carlos; Freitas, Ana Cristina
- Dark chocolate as a promising carrier for probiotic strainsPublication . Barbosa, Joana Cristina; Gomes, Ana Maria; Machado, Daniela; Silva, Mariana; Almeida, Diana; Andrade, José Carlos; Freitas, Ana Cristina
- Effect of emulsification/internal gelation-based microencapsulation on the viability of akkermansia muciniphila upon prolonged storage and simulated gastrointestinal passagePublication . Almeida, Diana; Machado, Daniela; Sousa, Sérgio; Seabra, Catarina Leal; Barbosa, Joana Cristina; Andrade, José Carlos; Gomes, Ana Maria; Freitas, Ana CristinaAkkermansia muciniphila is a common human intestinal commensal with a mucin-degrading nature. Its immunomodulatory characteristics and regulatory role of mucus layer and gut barrier integrity highlight the potential benefits of using this bacterium as an interventional player against inflammatory/cardio-metabolic disorders.In this work, we evaluate the effect of microencapsulation by the emulsification/internal gelation method onA. muciniphila survival during aerobic storage (0, 15, 30 and 95 days) and subsequent exposure to simulatedgastrointestinal passage, in comparison with that of free cells. The present results show that microencapsulation by internal gelation promotes a 64.4 % entrapment efficacy of A. muciniphila cells (maintaining a 108 orderof magnitude for cell viability). Moreover, physical characterization showed that microparticles mean size was53,5 ± 12,1 μm and, as observed by electron scanning microscopy, microcapsules were spherical in shape. Moreimportantly, as storage time increased, encapsulated A. muciniphila demonstrated higher stability in GI conditions, when compared to its free counterpart. In conclusion, microencapsulation by internal gelation seems tobe an appropriate strategy in protecting A. muciniphila against the detrimental gastrointestinal transit after longperiods of aerobic refrigerated storage.
- Evaluation of microencapsulation impact on akkermansia muciniphila’ culturability during freeze-drying, storage under different temperatures and atmospheric conditions, and in vitro gastrointestinal passagePublication . Almeida, Diana; Vedor, Rita; Barbosa, Joana Cristina; Machado, Daniela; Andrade, José; Gomes, Ana Maria; Freitas, Ana CristinaWith the surge of next-generation technologies the scientific community awareness to gut ecosystem importance on human health has been increasing. In the context of inflammatory and cardiometabolic disorders, which have major clinical/economic impact [1], the gut resident Akkermansia muciniphila emerges as a next-generation probiotic, due to its potential in their prevention/treatment [2][3]. However, the high sensitivity to acidic conditions and its aerotolerant metabolism hampers functional foods/nutraceuticals development [4]. To overcome such challenges, a combination of two cryoprotective agents was evaluated on the protection of microencapsulated A. muciniphila when exposed to detrimental conditions.
- Exploring freeze-drying as strategy to enhance viability of faecalibacterium duncaniae dsm 17677 upon aerobic storage and gastrointestinal conditionsPublication . Machado, Daniela; Domingos, Melany; Barbosa, Joana Cristina; Almeida, Diana; Andrade, José Carlos; Freitas, Ana Cristina; Gomes, Ana MariaFaecalibacterium duncaniae is an intestinal commensal bacterium proposed as a next-generation probiotic due to its promising outcomes in the treatment and prevention of several human diseases, which demonstrate its multiple contributions to the host’s health. However, its strict anaerobic nature has created several hurdles in the development of functional foods, nutraceuticals, and biotherapeutic products. Herein, we explored freeze-dried formulations containing prebiotics, cryoprotectants, and antioxidant agents as a technological strategy to enhance the viability of F. duncaniae DSM 17677 upon aerobic storage and gastrointestinal tract conditions. Our results indicate that freeze-dried F. duncaniae in a matrix containing inulin, sucrose, cysteine, and riboflavin survived at levels higher than 106 CFU/g and around 105 CFU/g after 1 and 4 days of aerobic storage at room temperature, respectively. Thus, the freeze-dried formulation with inulin, sucrose, cysteine, and riboflavin presents as a protective strategy to improve F. duncaniae viability under aerobic environments. Nevertheless, incorporation of a suitable coating aimed at protecting F. duncaniae against the detrimental gastrointestinal passage effects is urgently required, given its high susceptibility to extreme acidic pH values and bile.
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