Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6827
Título: Modeling the kinetics of whey protein hydrolysis brought about by enzymes from Cynara cardunculus
Autor: Barros, Rui M.
Malcata, F. Xavier
Palavras-chave: Enzymes
Dairy products
Kinetic Mechanism
Plant Proteases
Data: 2002
Editora: American Chemical Society
Citação: BARROS, Rui M.; MALCATA, F. Xavier - Hydrolysis Brought about by Enzymes from Cynara cardunculus. Journal of Agricultural and Food Chemistry. ISSN 0021-8561. Vol. 50 (2002), p. 4347-4356
Resumo: The purpose of this research work was to study the proteolytic activity of aqueous crude extracts of flowers of the plant Cynara cardunculus on the major whey proteins, namely, â-lactoglobulin (â-Lg) and R-lactalbumin (R-La). These extracts, containing a mixture of cardosins A and B (i.e., two distinct aspartic proteases), have been employed for many years in traditional cheese-making in Portugal and Spain. Cow’s milk sweet whey was incubated for up to 24 h at various ratios of addition of crude enzyme extract, under controlled pH (5.2 and 6.0) and temperature (55 °C). The samples collected were assayed by gel permeation chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A mechanistic model was proposed for the kinetics of the hydrolysis process, which is basically a double-substrate, double-enzyme Michaelis-Menten rate expression; the kinetic parameters were estimated by multiresponse, nonlinear regression analysis. The best estimates obtained for the specificity ratio (i.e., kcat/Km) of each cardosin within the mixture toward each whey protein indicated that said aspartic proteases possess a higher catalytic efficiency for R-La (0.42- 4.2 mM-1âs-1) than for â-Lg (0-0.064 mM-1âs-1), at least under the experimental conditions used. These ratios are below those previously reported for caseins and a synthetic hexapeptide. Cardosins are more active at pH 5.2 than at pH 6.0 and (as expected) at higher enzyme-to-substrate ratios.
Peer review: yes
URI: http://hdl.handle.net/10400.14/6827
Versão do Editor: http://pubs.acs.org/doi/abs/10.1021/jf0200319
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
Modeling the kinetics.pdf326,69 kBAdobe PDFVer/Abrir    Acesso Restrito. Solicitar cópia ao autor!

FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.