Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/5468
Título: Starting D-Optimal designs for batch kinetic studies of enzyme-catalyzed reactions in the presence of enzyme deactivation
Autor: Malcata, F. X.
Data: 1992
Editora: Wiley-Blackwell
Citação: MALCATA, F. X. - Starting D-Optimal designs for batch kinetic Studies of enzyme-catalyzed Reactions in the presence of enzyme deactivation. Biometrics. ISSN 0006-341. Vol. 48 n.º 3 (1992), p. 929-938
Resumo: This paper describes a strategy for the starting experimental design of experiments required by general research in the field of biochemical kinetics. The type of experiments that qualify for this analysis involve batch reactions catalyzed by soluble enzymes where the activity of the enzyme decays with time. Assuming that the catalytic action of the enzyme obeys a Michaelis-Menten rate expression and that the deactivation of the enzyme follows a first-order decay, the present analysis employs the dimensionless, integrated form of the overall rate expression to obtain a criterion (based on the maximization of the determinant of the derivative matrix) that relates the a priori estimates of the parameters with the times at which samples should be withdrawn from the reacting mixture. The analysis indicates that the initial concentration of substrate should be as large as possible, and that the samples should be taken at times corresponding to substrate concentrations of approximately 2/3, 1/4, and I/epsilon of the initial concentration (where epsilon should be as large as possible).
Peer review: yes
URI: http://hdl.handle.net/10400.14/5468
Versão do Editor: The definitive version is available at: http://www.jstor.org/pss/2532358
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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