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Advisor(s)
Abstract(s)
The release of fluorinated organic compounds from fire retardants or agrochemical products may have a
significant negative effect on soil ecosystems. In this study, the ability of Pisolithus tinctorius to tolerate
and degrade mono-fluorophenols (FP) was assessed. In vitro studies showed fungal growth in the presence
of 0.45 mM of 2-FP and 3-FP, but not in the presence of 4-FP. P. tinctorius was able to degrade up
to 79% and 92% of 1 mM 2-FP and 3-FP, respectively, in glucose supplemented liquid medium, suggesting
that 2- and 3-FP degradation occurred in co-metabolism with glucose consumption. 3-Fluorocatechol
(FC) and 4-FC were identified as metabolic intermediates using HPLC and LC–MS. Liberation of fluoride
was not detected suggesting that a fluorinated dead-end product was formed. In extracts of cells collected
at the end of cultures supplemented with the mono-FPs, a metabolic intermediate compatible with a
mass corresponding to a fluoromuconate compound, according to LC–MS data, was recovered. The results
further suggest that ectomycorrhizal fungi may be able to degrade mono-FP in pure culture while using
glucose as a carbon source, through a similar pathway as that found in bacteria. To our knowledge, this is
the first time that degradation of mono-FPs by an ectomycorrhizal fungus is reported.
Description
Keywords
Co-metabolism Mono-Fluorophenol Pisolithus tinctorius Fluorocatechol
Pedagogical Context
Citation
FRANCO, A. R.; SILVA FERREIRA, António César; CASTRO, Paula M. L. - Co-metabolic degradation of mono-fluorophenols by the ectomycorrhizal fungi Pisolithus tinctorius. Chemosphere. ISSN 0045-6535. Vol. 111 (2014), p. 260–265
Publisher
Elsevier