Epifluorescence microscope methods for bacterial enumeration in a 4-chlorophenol degrading consortium

Pacheco, C.C.; Alves, C.C.; Barreiros, L.; Castro, Paula M. L.; Teixeira, Paula

http://hdl.handle.net/10400.14/6835

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Abstract(s)

Epifluorescence microscope methods, namely BacLight, direct epifluorescence filter technique and Rhodamine 123, consistently underestimated plate bacterial counts in a 4-chlorophenol degrading consortium. Cells capable of passing through 0.2 mgrm filters, referred as `ultramicrocells', were found. Although cell counts were higher when traditional methods were used, BacLight and direct epifluorescence filter technique were convenient techniques for the systematic monitoring of bacteria involved in biodegradation processes, as results were consistent and available within a short time.

Keywords

Degrading consortia Fluorescence dyes Starvation Ultramicrocells Viable but non-culturable bacteria

URI

http://hdl.handle.net/10400.14/6835

Citation

PACHECO, C.C... [et al.] - Epifluorescence microscope methods for bacterial enumeration in a 4-chlorophenol degrading consortium. Biotechnology Letters. ISSN 1573-6776. Vol. 25, n.º 24 (2003) p. 2089-2092