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Effect of copper and zinc as sulfate or nitrate salts on soil microbiome dynamics and blaVIM-positive pseudomonas aeruginosa survival

2021-08-05Journal article Open access
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dc.contributor.authorFortunato, Gianuario
dc.contributor.authorVaz-Moreira, Ivone
dc.contributor.authorNunes, Olga C.
dc.contributor.authorManaia, Célia M.
dc.date.accessioned2021-06-22T10:13:51Z
dc.date.available2023-08-01T00:30:34Z
dc.date.issued2021-08-05
dc.description.abstractThe exposure of soil to metals and to antibiotic resistant bacteria may lead to the progressive deterioration of soil quality. The persistence of antibiotic resistant bacteria or antibiotic resistance genes in soil can be influenced by the microbial community or by soil amendments with metal salts. This work assessed the effect of soil amendment with copper and zinc, as sulfate or nitrate salts, on the fate of a carbapenem-resistant (blaVIM+) hospital effluent isolate of Pseudomonas aeruginosa (strain H1FC49) and on the variations of the microbial community composition. Microcosms with soil aged or not with copper and zinc salts (20 mM), and inoculated with P. aeruginosa H1FC49 were monitored at 0, 7, 14 and/or 30 days, for community composition (16S rRNA gene amplicon) and strain H1FC49 persistence. Data on culturable P. aeruginosa, quantitative PCR of the housekeeping gene ecf, and the presumably acquired genes blaVIM+ and integrase (intI1), and community composition were interpreted based on descriptive statistics and multivariate analysis. P. aeruginosa and the presumably acquired genes, were quantifiable in soil for up to one month, in both metal-amended and non-amended soil. Metal amendments were associated with a significant decrease of bacterial community diversity and richness. The persistence of P. aeruginosa and acquired genes in soils, combined with the adverse effect of metals on the bacterial community, highlight the vulnerability of soil to both types of exogenous contamination.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.doi10.1016/j.jhazmat.2021.125631pt_PT
dc.identifier.eid85103088738
dc.identifier.issn0304-3894
dc.identifier.pmid33773246
dc.identifier.urihttp://hdl.handle.net/10400.14/33828
dc.identifier.wos000657742400006
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectAntibiotic resistance gene persistencept_PT
dc.subjectBacteria survivalpt_PT
dc.subjectHeavy metalspt_PT
dc.subjectMetal saltspt_PT
dc.subjectSoil bacterial communitiespt_PT
dc.titleEffect of copper and zinc as sulfate or nitrate salts on soil microbiome dynamics and blaVIM-positive pseudomonas aeruginosa survivalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleJournal of Hazardous Materialspt_PT
oaire.citation.volume415pt_PT
person.familyNameVaz-Moreira
person.familyNameNunes
person.familyNameManaia
person.givenNameIvone
person.givenNameOlga
person.givenNameCélia
person.identifier602434
person.identifier.ciencia-id9E17-544E-800D
person.identifier.ciencia-idBA14-BD30-C9AF
person.identifier.ciencia-idC31F-553B-6365
person.identifier.orcid0000-0001-8912-9836
person.identifier.orcid0000-0003-4742-2537
person.identifier.orcid0000-0002-3674-1789
person.identifier.scopus-author-id16044425800
person.identifier.scopus-author-id7005088596
person.identifier.scopus-author-id6602465318
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf3e78f4a-1586-4e5c-a182-81009f20915a
relation.isAuthorOfPublication6b4a1abf-b245-41ad-8335-fa0f379650bb
relation.isAuthorOfPublicationa5f14577-0aed-4046-97da-5f4216c7aa07
relation.isAuthorOfPublication.latestForDiscoverya5f14577-0aed-4046-97da-5f4216c7aa07

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