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Biodegradation of diclofenac by the bacterial strain labrys portucalensis Fl 1

2018Journal article Restricted access
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dc.contributor.authorMoreira, Irina S.
dc.contributor.authorBessa, Vânia S.
dc.contributor.authorMurgolo, Sapia
dc.contributor.authorPiccirillo, Clara
dc.contributor.authorMascolo, Giuseppe
dc.contributor.authorCastro, Paula M.L.
dc.date.accessioned2018-09-13T12:01:13Z
dc.date.available2018-09-13T12:01:13Z
dc.date.issued2018
dc.description.abstractDiclofenac (DCF) is a widely used non-steroidal anti-inflammatory pharmaceutical which is detected in the environment at concentrations which can pose a threat to living organisms. In this study, biodegradation of DCF was assessed using the bacterial strain Labrys portucalensis F11. Biotransformation of 70% of DCF (1.7–34 μM), supplied as the sole carbon source, was achieved in 30 days. Complete degradation was reached via co-metabolism with acetate, over a period of 6 days for 1.7 µM and 25 days for 34 μM of DCF. The detection and identification of biodegradation intermediates was performed by UPLC-QTOF/MS/MS. The chemical structure of 12 metabolites is proposed. DCF degradation by strain F11 proceeds mainly by hydroxylation reactions; the formation of benzoquinone imine species seems to be a central step in the degradation pathway. Moreover, this is the first report that identified conjugated metabolites, resulting from sulfation reactions of DCF by bacteria. Stoichiometric liberation of chlorine and no detection of metabolites at the end of the experiments are strong indications of complete degradation of DCF by strain F11. To the best of our knowledge this is the first report that points to complete degradation of DCF by a single bacterial strain isolated from the environment.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMoreira, I. S., Bessa, V. S., Murgolo, S., Piccirillo, C., Mascolo, G., Castro, P. M. L. (2018). Biodegradation of Diclofenac by the bacterial strain Labrys portucalensis F11. Ecotoxicology and Environmental Safety, 152, 104–113pt_PT
dc.identifier.doi10.1016/j.ecoenv.2018.01.040pt_PT
dc.identifier.eid85041385870
dc.identifier.eissn1090-2414
dc.identifier.issn0147-6513
dc.identifier.pmid29407776
dc.identifier.urihttp://hdl.handle.net/10400.14/25687
dc.identifier.wos000427807100013
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCEQUIMED-Pest- OE/SAU/U14040/2014
dc.relationPEst-OE/EQB/LA0016/2013UID/Multi/50016/2013
dc.relation0022729
dc.relationFLUOROPHARMA - Biodegradation and removal of chiral and achiral fluorinated pharmaceuticals from wastewaters
dc.subjectDiclofenacpt_PT
dc.subjectLabrys portucalensis F11pt_PT
dc.subjectBiodegradationpt_PT
dc.subjectMetabolitespt_PT
dc.subjectCo-metabolismpt_PT
dc.titleBiodegradation of diclofenac by the bacterial strain labrys portucalensis Fl 1pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleFLUOROPHARMA - Biodegradation and removal of chiral and achiral fluorinated pharmaceuticals from wastewaters
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F87251%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F90146%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F86483%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEBB-EBI%2F111699%2F2009/PT
oaire.citation.endPage113
oaire.citation.startPage104
oaire.citation.titleEcotoxicology and Environmental Safetypt_PT
oaire.citation.volume152
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
oaire.fundingStream3599-PPCDT
person.familyNameMoreira
person.familyNameBessa
person.familyNamePiccirillo
person.familyNameMascolo
person.familyNameCastro
person.givenNameIrina Susana Sousa
person.givenNameVânia
person.givenNameClara
person.givenNameGiuseppe
person.givenNamePaula
person.identifierK-5841-2014
person.identifier962551
person.identifier2013444
person.identifier.ciencia-id161E-A350-7FDE
person.identifier.ciencia-id391B-75ED-6983
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.orcid0000-0001-6516-0994
person.identifier.orcid0000-0002-2676-7184
person.identifier.orcid0000-0002-6339-716X
person.identifier.orcid0000-0002-7858-2114
person.identifier.orcid0000-0001-8841-6606
person.identifier.ridK-5841-2014
person.identifier.ridK-8029-2014
person.identifier.ridN-8206-2013
person.identifier.ridK-3240-2012
person.identifier.ridM-8241-2013
person.identifier.scopus-author-id36798094100
person.identifier.scopus-author-id35214245200
person.identifier.scopus-author-id7004621626
person.identifier.scopus-author-id7102781782
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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