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Enantioselective HPLC analysis and biodegradation of atenolol, metoprolol and fluoxetine

2013Journal article Restricted access
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dc.contributor.authorRibeiro, Ana R.
dc.contributor.authorAfonso, Carlos M.
dc.contributor.authorCastro, Paula M. L.
dc.contributor.authorTiritan, Maria E.
dc.date.accessioned2013-05-03T10:20:39Z
dc.date.available2013-05-03T10:20:39Z
dc.date.issued2013
dc.description.abstractThe accurate quantification of enantiomers is crucial for assessing the biodegradation of chiral pharmaceuticals in the environment. Methods to quantify enantiomers in environmental matrices are scarce. Here, we used an enantioselective method, high-performance liquid chromatography with fluorescence detection (HPLC-FD), to analyze two beta-blockers, metoprolol and atenolol, and the antidepressant fluoxetine in an activated sludge consortium from a wastewater treatment plant. The vancomycin-based chiral stationary phase was used under polar ionic mode to achieve the enantioseparation of target chiral pharmaceuticals in a single chromatographic run. The method was successfully validated over a concentration range of 20–800 ng/mL for each enantiomer of both beta-blockers and of 50–800 ng/mL for fluoxetine enantiomers. The limits of detection were between 5 and 20 ng/mL and the limits of quantification were between 20 and 50 ng/mL, for all enantiomers. The intra- and inter-batch precision was lower than 5.66 and 8.37 %, respectively. Accuracy values were between 103.03 and 117.92 %, and recovery rates were in the range of 88.48–116.62 %. Furthermore, the enantioselective biodegradation of atenolol, metoprolol and fluoxetine was followed during 15 days. The (S)-enantiomeric form of metoprolol was degraded at higher extents, whereas the degradation of atenolol and fluoxetine did not show enantioselectivity under the applied conditions.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationRIBEIRO, Ana R. ...[et al.] - Enantioselective HPLC analysis and biodegradation of atenolol, metoprolol and fluoxetine. Environmental Chemistry Letters. ISSN 1610-3661. Vol. 11, n.º 1 (2013), p. 83-90por
dc.identifier.doi10.1007/s10311-012-0383-1
dc.identifier.eissn1610-3661
dc.identifier.issn1610-3653
dc.identifier.urihttp://hdl.handle.net/10400.14/10420
dc.identifier.wosWOS:000315491900012
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relation09-GCQF-CICS-09
dc.relationFLUOROPHARMA - Biodegradation and removal of chiral and achiral fluorinated pharmaceuticals from wastewaters
dc.relationStrategic Project - LA 16 - 2011-2012
dc.subjectPharmaceuticalspor
dc.subjectChiral ecotoxicitypor
dc.subjectEnantioselective biodegradationpor
dc.subjectHPLC-FDpor
dc.subjectPolar ionic modepor
dc.subjectChirobiotic Vpor
dc.titleEnantioselective HPLC analysis and biodegradation of atenolol, metoprolol and fluoxetinepor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleFLUOROPHARMA - Biodegradation and removal of chiral and achiral fluorinated pharmaceuticals from wastewaters
oaire.awardTitleStrategic Project - LA 16 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F64999%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEBB-EBI%2F111699%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0016%2F2011/PT
oaire.citation.endPage90
oaire.citation.issue1
oaire.citation.startPage83
oaire.citation.titleEnvironmental Chemistry Letters
oaire.citation.volume11
oaire.fundingStreamSFRH
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRibeiro
person.familyNameAfonso
person.familyNameCastro
person.familyNameTiritan
person.givenNameAna Rita Lado
person.givenNameCarlos
person.givenNamePaula
person.givenNameMaria Elizabeth
person.identifier903786
person.identifier2013444
person.identifier1310409
person.identifier.ciencia-id421D-DC6E-F6E3
person.identifier.ciencia-id6A10-3565-6158
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.ciencia-idD915-BFC0-FB3A
person.identifier.orcid0000-0002-4445-5379
person.identifier.orcid0000-0002-5731-5789
person.identifier.orcid0000-0001-8841-6606
person.identifier.orcid0000-0003-3320-730X
person.identifier.ridI-5989-2015
person.identifier.ridM-8241-2013
person.identifier.ridK-9492-2014
person.identifier.scopus-author-id57207918943
person.identifier.scopus-author-id36145043600
person.identifier.scopus-author-id7102781782
person.identifier.scopus-author-id6602890981
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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