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Structure and function of a novel antioxidant peptide from the skin of tropical frogs

2018Journal article Restricted access
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dc.contributor.authorBarbosa, Eder Alves
dc.contributor.authorOliveira, Ana
dc.contributor.authorPlácido, Alexandra
dc.contributor.authorSocodato, Renato
dc.contributor.authorPortugal, Camila C.
dc.contributor.authorMafud, Ana Carolina
dc.contributor.authorLeite, José Roberto S.A.
dc.contributor.authorPintado, M. E.
dc.date.accessioned2018-09-17T16:27:22Z
dc.date.available2018-09-17T16:27:22Z
dc.date.issued2018
dc.description.abstractThe amphibian skin plays an important role protecting the organism from external harmful factors such as microorganisms or UV radiation. Based on biorational strategies, many studies have investigated the cutaneous secretion of anurans as a source of bioactive molecules. By a peptidomic approach, a novel antioxidant peptide (AOP) with in vitro free radical scavenging ability was isolated from Physalaemus nattereri. The AOP, named antioxidin-I, has a molecular weight [M+H]+ = 1543.69 Da and a TWYFITPYIPDK primary amino acid sequence. The gene encoding the antioxidin-I precursor was expressed in the skin tissue of three other Tropical frog species: Phyllomedusa tarsius, P. distincta and Pithecopus rohdei. cDNA sequencing revealed highly homologous regions (signal peptide and acidic region). Mature antioxidin-I has a novel primary sequence with low similarity compared with previously described amphibian's AOPs. Antioxidin-I adopts a random structure even at high concentrations of hydrophobic solvent, it has poor antimicrobial activity and poor performance in free radical scavenging assays in vitro, with the exception of the ORAC assay. However, antioxidin-I presented a low cytotoxicity and suppressed menadione-induced redox imbalance when tested with fibroblast in culture. In addition, it had the capacity to substantially attenuate the hypoxia-induced production of reactive oxygen species when tested in hypoxia exposed living microglial cells, suggesting a potential neuroprotective role for this peptide.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBarbosa, E.A., Oliveira, A., Plácido, A., Socodato, R., Portugal, C.C., Mafud, A.C., …Leite, J.R.S.A. (2018). Structure and function of a novel antioxidant peptide from the skin of tropical frogs. Free Radical Biology and Medicine, 115, 68–79pt_PT
dc.identifier.doi10.1016/j.freeradbiomed.2017.11.001pt_PT
dc.identifier.eid85034997591
dc.identifier.eissn1873-4596
dc.identifier.issn0891-5849
dc.identifier.pmid29162516
dc.identifier.urihttp://hdl.handle.net/10400.14/25703
dc.identifier.wos000419709200007
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationIF/00092/2014
dc.relation2014/02282-6
dc.relation2016/18023-5
dc.relationPQ-Sr 306036/2016-9
dc.relation1603966
dc.subjectAmphibiapt_PT
dc.subjectAntioxidant peptidept_PT
dc.subjectAntioxidinpt_PT
dc.subjectMicrogliapt_PT
dc.subjectReactive oxygen speciespt_PT
dc.subjectSkin secretionpt_PT
dc.titleStructure and function of a novel antioxidant peptide from the skin of tropical frogspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F97995%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04378%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F91962%2F2012/PT
oaire.citation.endPage79
oaire.citation.startPage68
oaire.citation.titleFree Radical Biology and Medicinept_PT
oaire.citation.volume115
oaire.fundingStreamSFRH
oaire.fundingStream5876
oaire.fundingStream5876
oaire.fundingStreamSFRH
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person.familyNameOliveira
person.familyNamePlácido
person.familyNameSocodato
person.familyNamePortugal
person.familyNameLeite
person.familyNamePintado
person.givenNameAna
person.givenNameAlexandra
person.givenNameRenato
person.givenNameCamila
person.givenNameJosé Roberto
person.givenNameMaria Manuela
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person.identifier.scopus-author-id55937245800
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project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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