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Physical processing or supplementation of feeds with phytogenic compounds, alginate oligosaccharide or nucleotides as methods to improve the utilization of Gracilaria gracilis by juvenile European seabass (Dicentrarchus labrax)

2021Journal article Restricted access
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dc.contributor.authorValente, Luisa M.P.
dc.contributor.authorBatista, Sónia
dc.contributor.authorRibeiro, Catarina
dc.contributor.authorPereira, Ricardo
dc.contributor.authorOliveira, Beatriz
dc.contributor.authorGarrido, Inês
dc.contributor.authorBaião, Luís F.
dc.contributor.authorTulli, Francesa
dc.contributor.authorMessina, Maria
dc.contributor.authorPierre, Ronan
dc.contributor.authorAbreu, Helena
dc.contributor.authorPintado, Manuela
dc.contributor.authorKiron, Viswanath
dc.date.accessioned2021-01-08T12:24:20Z
dc.date.available2021-01-08T12:24:20Z
dc.date.issued2021
dc.description.abstractThis study assessed both the effectiveness of a physical-mechanical rupture method and the ability of feed additives (phytogenic compounds, alginate oligosaccharide and nucleotides) to enhance the utilization of G. gracilis by European seabass. A commercial-based diet was used as control diet (CTRL) and compared with five isoproteic (53.5% Dry matter, DM) and isolipidic (14.9% DM) diets containing 8% of G. gracilis. This seaweed was either unprocessed (diet GRA) or subjected to physical processing (diet GRAP). The three additive-containing diets were formulated by supplementing the GRA diet with either 0.02% phytogenic compounds (PHY), 2.5% oligo-alginate (OAS) or 0.08% free nucleotides (NUC). Triplicate groups of nineteen fish (29.7 ± 0.02 g) were distributed by 50 L tanks (11.3 kg m−3) and fed the experimental diets to satiety during 106 days. By the end of the trial, growth performance and nutrient utilization (specific growth ratio, feed conversion ratio, apparent digestibility coefficients, nutrient balance, intestinal brush border membrane enzyme activities and plasma metabolic parameters), gut histomorphology, antioxidant and immunological status of fish were evaluated. The ability of fish to digest seaweed-rich diets was largely improved by the technological processing of G. gracilis, albeit nil effect on fish specific growth rate (1.0 in all groups). This major achievement was associated with increased ability of GRAP to digest protein (84 vs 68% in GRA) and energy (64 vs 38% in GRA). The use of feed additives in Gracilaria-rich diets was less efficacious in improving European sea bass nutrient and energy ADCs, but have still improved the overall digestibility of those diets. Fish fed alginate oligosaccharide was mainly associated with increased activity of anterior intestine enzymes, particularly intestinal alkaline phosphatase (IAP; 174.4 vs 104.7–120.6 μm min−1 g−1 in Gracilaria-rich diets). Moreover, the algae technological processing and both the nucleotides and the alginate oligosaccharide seem to have positively affected the intestinal villus width compared to the negative impact seen in fish fed GRA. The tested additives had limited impact on oxidative stress, although glutathione peroxidase (GPx; 2.1 μmol min−1 mg protein−1) and catalase (CAT; 35 μmol min−1 mg protein−1) activities were lowest in fish fed NUC and PHY, respectively. It can be concluded that the physical processing of Gracilaria sp. or the addition of either oligo-alginate or nucleotides can effectively increase the nutritional value of this seaweed for European seabass diets.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationValente, L.M.P., Batista, S., Ribeiro, C., Pereira, R., Oliveira, B., Garrido, I., Baião, L.F., ..., Kiron, V.. (2021). Physical processing or supplementation of feeds with phytogenic compounds, alginate oligosaccharide or nucleotides as methods to improve the utilization of Gracilaria gracilis by juvenile European seabass (Dicentrarchus labrax). Aquaculture, 530:735914pt_PT
dc.identifier.doi10.1016/j.aquaculture.2020.735914pt_PT
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10400.14/31608
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.relationCentre for Biotechnology and Fine Chemistry
dc.subjectAlgae nutrient bioavailabilitypt_PT
dc.subjectCell disruption methodspt_PT
dc.subjectFeed additivespt_PT
dc.subjectIntestinal morphologypt_PT
dc.subjectDigestive enzymespt_PT
dc.titlePhysical processing or supplementation of feeds with phytogenic compounds, alginate oligosaccharide or nucleotides as methods to improve the utilization of Gracilaria gracilis by juvenile European seabass (Dicentrarchus labrax)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardTitleCentre for Biotechnology and Fine Chemistry
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04423%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04423%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F50016%2F2019/PT
oaire.citation.titleAquaculturept_PT
oaire.citation.volume530pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameValente
person.familyNameBatista
person.familyNameBaião
person.familyNamePintado
person.givenNameLuísa
person.givenNameSónia
person.givenNameLuís Francisco
person.givenNameMaria Manuela
person.identifier159662
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person.identifier.ciencia-id7315-83FA-4957
person.identifier.ciencia-idCD19-22F9-B5C0
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0002-2496-4854
person.identifier.orcid0000-0002-4591-8873
person.identifier.orcid0000-0002-8532-5132
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridA-3769-2010
person.identifier.ridB-9150-2017
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id7005825980
person.identifier.scopus-author-id52563285200
person.identifier.scopus-author-id7004483898
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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