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Combination of PLGA nanoparticles with mucoadhesive guar-gum films for buccal delivery of antihypertensive peptide

2018Journal article Restricted access
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dc.contributor.authorCastro, Pedro M.
dc.contributor.authorBaptista, Patrícia
dc.contributor.authorMadureira, Ana Raquel
dc.contributor.authorSarmento, Bruno
dc.contributor.authorPintado, Manuela E.
dc.date.accessioned2018-09-13T16:54:38Z
dc.date.available2018-09-13T16:54:38Z
dc.date.issued2018
dc.description.abstractOral administration of proteins and peptides still is a challenging task to overcome due to low permeability through absorptive epithelia, degradation and metabolism that lead to poor bioavailability. Attempting to overcome such limitations, an antihypertensive peptide derived from whey protein, with KGYGGVSLPEW sequence, was incorporated for the first time into polymeric nanoparticles. An experimental design was followed in order to optimize drug-loading, association efficiency, mean particle size, zeta-potential and polydispersity index of a formulation of poly(lactic-co-glycolic acid) (PLGA) nanoparticles as carriers for bioactive peptides. In sequence, peptide-loaded PLGA nanoparticles were incorporated in a guar-gum film matrix, resulting in a combined delivery system aiming to promote slow release and permeation across buccal epithelium. Neither PLGA nanoparticles, guar-gum films nor the conjugation of PLGA nanoparticles and guar-gum films (GfNp) significantly compromised in vitro TR146 human buccal carcinoma cell line viability after 12 h contact, as assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide reduction assay (MTT). In vitro release assay for developed formulations allowed to conclude that the combination of orodispersible film and nanoparticles granted a slower release of AhP when compared with PLGA or guar-gum films alone or with control. GfNp offered more effective, synergistic, in vitro permeation of TR146 cell multilayer in comparison with guar-gum films or PLGA nanoparticles alone. The combination of PLGA nanoparticles with guar-gum films represent a suitable alternative to conventional per os delivery systems, leading to an increased buccal permeability of carried antihypertensive peptide.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCastro, P., Baptista, P., Madureira, R., Sarmento, B., Pintado, M. E. (2018). Combination of PLGA nanoparticles with mucoadhesive guar-gum films for buccal delivery of antihypertensive peptide. International Journal of Pharmaceutics, 547, 593–601pt_PT
dc.identifier.doi10.1016/j.ijpharm.2018.05.051pt_PT
dc.identifier.eid85048820179
dc.identifier.eissn1873-3476
dc.identifier.issn0378-5173
dc.identifier.pmid29800740
dc.identifier.urihttp://hdl.handle.net/10400.14/25688
dc.identifier.wos000439096500059
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationNORTE-01-0145-FEDER-000012
dc.relationDevelopment of oral strips containing nanoencapsulated bioactive compounds
dc.relationPOCI-01-0145-FEDER-007274
dc.subjectAntihypertensive peptidept_PT
dc.subjectBuccal deliverypt_PT
dc.subjectGuar-gum filmspt_PT
dc.subjectPLGA nanoparticlespt_PT
dc.subjectWhey proteinpt_PT
dc.titleCombination of PLGA nanoparticles with mucoadhesive guar-gum films for buccal delivery of antihypertensive peptidept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDevelopment of oral strips containing nanoencapsulated bioactive compounds
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBBB-NAN%2F3249%2F2014/PT
oaire.citation.endPage601
oaire.citation.issue1-2
oaire.citation.startPage593
oaire.citation.titleInternational Journal of Pharmaceuticspt_PT
oaire.citation.volume574
oaire.fundingStream3599-PPCDT
person.familyNameMadureira
person.familyNameSarmento
person.familyNamePintado
person.givenNameRaquel
person.givenNameBruno
person.givenNameMaria Manuela
person.identifierM-4659-2013
person.identifier152609
person.identifier456608
person.identifier.ciencia-id1C14-25F8-CC07
person.identifier.ciencia-idE816-B6B0-06A5
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0001-6596-1927
person.identifier.orcid0000-0001-5763-7553
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridM-4659-2013
person.identifier.ridJ-6265-2013
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id8384097900
person.identifier.scopus-author-id57194334236
person.identifier.scopus-author-id7004483898
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationd33a1d99-423e-4ecd-ae9e-b3e46262b521
relation.isAuthorOfPublication1df2ac4c-2a82-4f3b-9aea-6f4fb9964752
relation.isAuthorOfPublicationba387c7d-27c9-4016-895c-b35597e91ebc
relation.isAuthorOfPublication.latestForDiscoveryd33a1d99-423e-4ecd-ae9e-b3e46262b521
relation.isProjectOfPublication9681d077-5bd3-4a82-830f-a9a684b77839
relation.isProjectOfPublication.latestForDiscovery9681d077-5bd3-4a82-830f-a9a684b77839

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