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Advisor(s)
Abstract(s)
The environment is the original and most ancient source of the antibiotic resistance determinants that threat the
human health nowadays. In the environment, water is a privileged habitat and mode of dissemination of bacteria
of different origins. Freshwater bodies that cross urban areas are supposed to hold a complex mixture of both
human/animal origin and strictly environmental bacteria. In this study,wewere interested in unveiling the bacterial
diversity in urban river transects and, simultaneously, investigate the occurrence of antibiotic resistant bacteria,
in particular themultidrug resistant (MDR).With this aim, water and sediments of two rivers were sampled from
an urban transect and the bacterial diversitywas assessed based on 16S rRNA gene-based community analysis and,
simultaneously, total heterotrophic bacteria were isolated in the presence and in the absence of antibiotics.
The three predominant phyla were Proteobacteria, Bacteroidetes and Actinobacteria, in water, or Acidobacteria, in
sediments. MDR bacteria were observed to belong to the predominant phyla observed in water, mostly of the
classes Gamma- and Betaproteobacteria (Proteobacteria) and Sphingobacteriia and Flavobacteriia (Bacteroidetes)
and belonged to genera of ubiquitous (Pseudomonas, Acinetobacter, Stenotrophomonas) ormainly environmental
(Chitinophaga, Chryseobacterium) bacteria. The observation that MDR bacteria are widespread in the environment
and over distinct phylogenetic lineages has two relevant implications: i) the potential of environmental
bacteria as source or facilitators for antibiotic resistance acquisition; ii) the need to complement cultureindependent
methods with culture-based approaches in order to identify major sources of MDR profiles.
Description
Keywords
Environmental bacteria Natural antibiotic resistance Contaminant antibiotic resistance Bacterial diversity
Pedagogical Context
Citation
NARCISO-DA-ROCHA, Carlos; MANAIA, C. M. - Multidrug resistance phenotypes are widespread over different bacterial taxonomic groups thriving in surface water. Science of the Total Environment. ISSN 0048-9697. Vol. 563 (2016), p. 1-9
Publisher
Elsevier