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High frequency of the EMRSA-15 clone (ST22-MRSA-IV) in hospital wastewater

2022-01Journal article Open access
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dc.contributor.authorSilva, Vanessa
dc.contributor.authorRibeiro, Jessica
dc.contributor.authorRocha, Jaqueline
dc.contributor.authorManaia, Célia M.
dc.contributor.authorSilva, Adriana
dc.contributor.authorPereira, José Eduardo
dc.contributor.authorMaltez, Luís
dc.contributor.authorCapelo, José Luis
dc.contributor.authorIgrejas, Gilberto
dc.contributor.authorPoeta, Patrícia
dc.date.accessioned2022-01-25T10:40:44Z
dc.date.available2022-01-25T10:40:44Z
dc.date.issued2022-01
dc.description.abstractHospital wastewaters often carry multidrug-resistant bacteria and priority pathogens, such as methicillin-resistant Staphylococcus aureus (MRSA). Pathogens and antibiotic resistance genes present in wastewaters may reach the natural environment facilitating their spread. Thus, we aimed to isolate MRSA from wastewater of 3 hospitals located in the north of Portugal and to characterize the isolates regarding the antimicrobial resistance and genetic lineages. A total of 96 wastewater samples were collected over six months. The water was filtered, and the filtration membrane was immersed in BHI broth supplemented with 6.5% of NaCl and incubated. The inoculum was streaked in ORSAB agar plates for MRSA isolation. The isolates susceptibility testing was performed against 14 antimicrobial agents. The presence of resistance and virulence genes was accessed by PCR. Molecular typing was performed in all isolates. From the 96 samples, 28 (29.2%) were MRSA-positive. Most isolates had a multidrug-resistant profile and carried the mecA, blaZ, aac(6′)-Ie-aph(2′′)-Ia, aph(3′)-IIIa, ermA, ermB, ermC, tetL, tetM, dfrA dfrG and catpC221 genes. Most of the isolates were ascribed to the immune evasion cluster (IEC) type B. The isolates belonged to ST22-IV, ST8-IV and ST105-II and spa-types t747, t1302, t19963, t6966, t020, t008 and tOur study shows that MRSA can be found over time in hospital wastewater. The wastewater treatment processes can reduce the MRSA load. The great majority of the isolates belonged to ST22 and spa-type t747 which suggests the fitness of these genetic lineages in hospital effluents.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/microorganisms10010147pt_PT
dc.identifier.eid85122709805
dc.identifier.issn2076-2607
dc.identifier.pmcPMC8780076
dc.identifier.pmid35056595
dc.identifier.urihttp://hdl.handle.net/10400.14/36553
dc.identifier.wos000747763800001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEffluentspt_PT
dc.subjectEMRSA-15pt_PT
dc.subjectHospitalpt_PT
dc.subjectMRSApt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.subjectWastewaterspt_PT
dc.titleHigh frequency of the EMRSA-15 clone (ST22-MRSA-IV) in hospital wastewaterpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume10pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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