Repository logo
 
Publication

Biosafety measures for Alicyclobacillus spp. strains across various levels of biohazard

2024-09Journal article Open access
Simple item page
dc.contributor.authorSajid, Marina
dc.contributor.authorMehmood, Sajid
dc.contributor.authorYuan, Yahong
dc.contributor.authorYue, Tianli
dc.contributor.authorKhalid, Muhammad Zubair
dc.contributor.authorMujtaba, Ahmad
dc.contributor.authorAlharbi, Sulaiman Ali
dc.contributor.authorAnsari, Mohammad Javed
dc.contributor.authorZinedine, Abdellah
dc.contributor.authorRocha, João Miguel
dc.date.accessioned2024-07-10T07:23:41Z
dc.date.available2024-07-10T07:23:41Z
dc.date.issued2024-09
dc.description.abstractAlicyclobacillus bacteria are important contaminants in the beverage industry because their spores remain in the product after usual pasteurization. At the same time, their impact on human health has yet to be characterized, as it is generally assumed to be low or non-existent. However, these bacteria are causing quality concerns mainly due to odor and taste changes of the product. Since potential health effects are not precisely known, an experimental assessment was performed, including a biosafety assessment of six viable and non-viable vegetative and spore forms of Alicyclobacillus spp. strains using cell cultures and rodent study. The monolayer of Caco-2 (Cancer coli-2) cells was investigated for its adsorption effect on the epithelium of the small intestine of mice. Lactate dehydrogenase leakage (LDH) and transepithelial electrical resistance (TEER) tests were used to ensure the integrity of the cell membrane and tight junctions. The methylthiazole tetrazolium bromide (MTT) assay examined in vitro cytotoxicity in Caco-2 and HepG2 cell lines. The hemolysis of erythrocytes was spectrophotometrically measured. The results showed negligible cytotoxicity or non-toxic response in mice. In conclusion, Alicyclobacillus spp. exhibited biocompatibility with negligible cytotoxicity and minimal safety concerns.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.fct.2024.114840pt_PT
dc.identifier.eid85197214437
dc.identifier.issn0278-6915
dc.identifier.pmid38944144
dc.identifier.urihttp://hdl.handle.net/10400.14/45719
dc.identifier.urnurn:E0C1FC4EFF29D9B7BC683B1220A24D02
dc.identifier.wos001265378800001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBiosafetypt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectTransepithelial electrical resistance (TEER)pt_PT
dc.subjectMethylthiazole tetrazolium bromide (MTT) assaypt_PT
dc.subjectLactate dehydrogenase (LDH) leakagept_PT
dc.subjectBiohazardspt_PT
dc.subjectAlicyclobacilluspt_PT
dc.titleBiosafety measures for Alicyclobacillus spp. strains across various levels of biohazardpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleFood and Chemical Toxicologypt_PT
oaire.citation.volume191pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
101670510.pdf
Size:
14.85 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.44 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CBQF - Contribuições em Revistas Científicas / Contribution to Journals