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Film-nanoparticle composite for enhanced oral delivery of alpha-casozepine

2019Journal article Open access
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dc.contributor.authorCastro, Pedro M.
dc.contributor.authorBaptista, Patricia
dc.contributor.authorZuccheri, Giampaolo
dc.contributor.authorMadureira, Ana Raquel
dc.contributor.authorSarmento, Bruno
dc.contributor.authorPintado, Manuela E.
dc.date.accessioned2019-06-07T11:52:29Z
dc.date.available2019-06-07T11:52:29Z
dc.date.issued2019
dc.description.abstractWhey-derived alpha-casozepine bioactive peptide (YLGYLEQLLR) was associated with previously optimized guar-gum film-PLGA nanoparticles, aiming to increase both stability across gastrointestinal tract and permeability across absorptive epithelia. Oral films associated with nanoparticles (FNp) enhance buccal absorption along with protection of carried bioactive molecules that are swallowed, with inherent increase of bioavailability. None of developed formulations induced significant loss of cell viability. Permeability across both buccal and intestinal cell barriers was enhanced when alpha-casozepine was carried by FNp system, when compared with film and nanoparticles alone, in a simulated gastrointestinal tract environment. Moreover, differences in permeability profile across buccal and intestinal epithelia were in accordance with the slower erosion of PLGA nanoparticles in a media of neutral pH, resembling oral cavity conditions, and a faster erosion in acidic conditions, as occurs in stomach, as observed by a continuous analysis of nanoparticle morphology over 980 min by atomic force microscopy. Additionally, apparent permeability of alpha-casozepine across TR146 human buccal carcinoma cells and Caco-2/HT29-MTX co-culture, carried by FNp was indeed superior when compared with peptide loaded in PLGA nanoparticles and in films alone or with free peptide control solution. Both FNp and PLGA nanoparticles alone enhanced the permeability of relaxing peptide compared with guar-gum films alone. An increased tongue adhesion when PLGA nanoparticles were added to the guar-gum films was also observed. Developed formulations improved both buccal an intestinal absorption of carried bioactive molecules without compromising cell viability.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationCastro, P. M., Baptista, P., Zuccheri, G., Madureira, A. R., Sarmento, B., & Pintado, M. E. (2019). Film-nanoparticle composite for enhanced oral delivery of alpha-casozepine. Colloids and Surfaces B: Biointerfaces, 181, 149–157. https://doi.org/10.1016/j.colsurfb.2019.05.029pt_PT
dc.identifier.doi10.1016/j.colsurfb.2019.05.029pt_PT
dc.identifier.eid85065906663
dc.identifier.eissn1873-4367
dc.identifier.issn0927-7765
dc.identifier.pmid31128515
dc.identifier.urihttp://hdl.handle.net/10400.14/27730
dc.identifier.wos000481565300020
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationNORTE-08-5369-FSE-000007
dc.relationDevelopment of oral strips containing nanoencapsulated bioactive compounds
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAlpha-Casozepinept_PT
dc.subjectPLGA nanoparticlespt_PT
dc.subjectOral filmspt_PT
dc.subjectBuccal deliverypt_PT
dc.subjectOral deliverypt_PT
dc.titleFilm-nanoparticle composite for enhanced oral delivery of alpha-casozepinept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDevelopment of oral strips containing nanoencapsulated bioactive compounds
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBBB-NAN%2F3249%2F2014/PT
oaire.citation.endPage157
oaire.citation.startPage149
oaire.citation.titleColloids and Surfaces B: Biointerfacespt_PT
oaire.citation.volume181
oaire.fundingStream3599-PPCDT
person.familyNameMiranda de Castro
person.familyNameBatista
person.familyNameZuccheri
person.familyNameMadureira
person.familyNameSarmento
person.familyNamePintado
person.givenNamePedro João
person.givenNamePatrícia
person.givenNameGiampaolo
person.givenNameRaquel
person.givenNameBruno
person.givenNameMaria Manuela
person.identifierAAF-7021-2020
person.identifierM-4659-2013
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person.identifier.orcid0000-0001-7164-8005
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person.identifier.orcid0000-0003-4777-5391
person.identifier.orcid0000-0001-6596-1927
person.identifier.orcid0000-0001-5763-7553
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridI-7378-2012
person.identifier.ridM-4659-2013
person.identifier.ridJ-6265-2013
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id57201808340
person.identifier.scopus-author-id37092408800
person.identifier.scopus-author-id6601943692
person.identifier.scopus-author-id8384097900
person.identifier.scopus-author-id57194334236
person.identifier.scopus-author-id7004483898
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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