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Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repair

2023-08Journal article Open access
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dc.contributor.authorMachado, Alexandra
dc.contributor.authorPereira, Isabel
dc.contributor.authorPereira, José Eduardo
dc.contributor.authorMaltez, Luís
dc.contributor.authorBrandão, Ana
dc.contributor.authorAlvites, Rui
dc.contributor.authorSousa, Ana Catarina
dc.contributor.authorBranquinho, Mariana
dc.contributor.authorCaseiro, Ana Rita
dc.contributor.authorPedrosa, Sílvia Santos
dc.contributor.authorMaurício, Ana Colette
dc.contributor.authorPires, Isabel
dc.contributor.authorPrada, Justina
dc.contributor.authorSantos, José Domingos
dc.contributor.authorGama, Miguel
dc.date.accessioned2023-09-15T16:12:41Z
dc.date.available2023-09-15T16:12:41Z
dc.date.issued2023-08
dc.description.abstractRegeneration of severe bone defects remains a challenge. A formulation of synthetic glass-reinforced hydroxyapatite bone substitute, Bonelike® Poro (BL®P), 250–500 µm-diameter, with a dextrin-based hydrogel (HG), further loaded with human dental pulp stem cells (hDPSCs) with osteogenic differentiation ability, was tested for the management of critical-sized defects in an ovine model. Morphology, calcium release, and mechanical strength of HG + BL®P were analyzed. Then, BL®P, HG + BL®P, and 106 hDPSCs-loaded HG + BL®P were implanted in ovine critical-sized 14 mm-diameter calvaria defects. Bone samples were collected after 3 and 6 weeks for histological and micro-CT analysis. BL®P exhibits a suitable porous size for cell ingrowth, from the nm (>200 nm) to the µm (5 µm) range. The addition of BL®P granules to the HG resulted in increased compressive elastic modulus and ultimate tensile strength. The mildly acidic nature of the HG contributed to a faster dissolution of granules. In vivo results confirmed the HG suitability as a carrier, providing better defect filling, easy handling, and injectability of BL®P without compromising new bone formation nor biocompatibility. The HG + BL®P formulations can successfully regenerate critical-sized defects; however, addition of hDPSCs did not significantly enhance new bone formation under these conditions. Granular BL®P provides an effective alternative to autologous grafts. The HG acts as a biocompatible carrier of granular bone substitutes and cells, conferring injectability and cohesivity.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.mtla.2023.101859pt_PT
dc.identifier.eid85166467965
dc.identifier.issn2589-1529
dc.identifier.urihttp://hdl.handle.net/10400.14/42398
dc.identifier.wos001052629400001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBone regenerationpt_PT
dc.subjectDental pulppt_PT
dc.subjectDextrinpt_PT
dc.subjectHydrogelpt_PT
dc.subjectInjectablept_PT
dc.subjectStem cellspt_PT
dc.titleDextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repairpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleMaterialiapt_PT
oaire.citation.volume30pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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