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Fate of cefotaxime-resistant enterobacteriaceae and ESBL-producers over a full-scale wastewater treatment process with UV disinfection

2018Journal article Restricted access
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dc.contributor.authorSilva, Isabel
dc.contributor.authorTacão, Marta
dc.contributor.authorTavares, Rafael D.S.
dc.contributor.authorMiranda, Rita
dc.contributor.authorAraújo, Susana
dc.contributor.authorManaia, Célia M.
dc.contributor.authorHenriques, Isabel
dc.date.accessioned2018-09-17T16:05:02Z
dc.date.available2018-09-17T16:05:02Z
dc.date.issued2018
dc.description.abstractDisinfection by UV radiation is one of themost promising solutions to reduce the bacterial load and antibiotic resistance in the final effluents of urban wastewater treatment plants (UWTP). Our aim was to evaluate the fate of cefotaxime-resistant Enterobacteriaceae and Extended Spectrum Beta-Lactamase (ESBL) producers in a full-scale system that includes UV-C disinfection. Over treatment, the abundance of cefotaxime-resistant Enterobacteriaceaewas reduced,with reductions of 1.9 log units after secondary treatment (STWsamples) and 1.8 log following UV disinfection (UTW samples). These reductions, did not reflect the variations in the prevalence of cefotaximeresistant Enterobacteriaceae, estimated to be of 3% in rawwastewater (RW), 18% in STWand 3% in UTW. A significant increase of cefotaxime-resistant bacterial counts (0.5 log; p b 0.05)was observed after 3 days of storage. In a total of 1799 cefotaxime-resistant Enterobacteriaceae isolates, 15% harboured blaCTX-M (n = 274), 11% blaTEM (n = 194) and 4% blaSHV (n = 72). While the ESBL gene prevalence decreased over treatment, the prevalence of the intI1 gene decreased after ST but slightly increased in UTW samples. The blaCTX-M-carriers were identified as Escherichia coli and Klebsiella pneumoniae, mostly multi-drug resistant (90.5%) and carrying integrase genes (82.8%). The blaCTX-M gene variants (48 blaCTX-M-15, 9 blaCTX-M-32, 8 blaCTX-M-1, 5 blaCTX-M-27, and 2 blaCTX-M-14) were flanked by ISEcp1, ISEcp1/IS26, IS903 and ORF477 in 8 different arrangements. The IncF plasmid replicon type was highly prevalent among blaCTX-M-carrying Escherichia coli (74.5%) while IncR predominated among K. pneumoniae (54.5%). Our results confirmed the potential of UV-C disinfection to remove antibiotic resistant bacteria. Still, resistant Enterobacteriaceae (about 30 × 106 cells per m3 of water), presenting traits that might potentiate antibiotic resistance spread, are released in the final effluent. In addition, a significant regrowthwas observed after storage. These results suggest that improvements ofwastewater disinfection are still required tominimize the risks associated with UWTP dischargespt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, I., Tacão, M., Tavares, R.D.S., Miranda, R., Araújo, S., Manaia, C. M., Henriques, I. (2018). Fate of cefotaxime-resistant enterobacteriaceae and ESBL-producers over a full-scale wastewater treatment process with UV disinfection. Science of the Total Environment, 639, 1028–1037pt_PT
dc.identifier.doi10.1016/j.scitotenv.2018.05.229pt_PT
dc.identifier.eid85047305678
dc.identifier.eissn1879-1026
dc.identifier.issn0048-9697
dc.identifier.pmid29929272
dc.identifier.urihttp://hdl.handle.net/10400.14/25694
dc.identifier.wos000436806200102
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationIF/00492/2013pt_PT
dc.relationSFRH/114855/2016pt_PT
dc.relationSFRH/BD/52573/2014pt_PT
dc.relationUID/AMB/50017-POCI-01-0145-FEDER-007638pt_PT
dc.subjectUrban wastewater treatment plantspt_PT
dc.subjectUV-C disinfectionpt_PT
dc.subjectExtended spectrum beta-lactamases (ESBLs)pt_PT
dc.subjectblaCTX-Mpt_PT
dc.subjectEscherichia colipt_PT
dc.subjectKlebsiella pneumoniaept_PT
dc.titleFate of cefotaxime-resistant enterobacteriaceae and ESBL-producers over a full-scale wastewater treatment process with UV disinfectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/WaterJPI%2F0002%2F2013/PT
oaire.citation.endPage1037
oaire.citation.startPage1028
oaire.citation.titleScience of the Total Environmentpt_PT
oaire.citation.volume639
oaire.fundingStream3599-PPCDT
person.familyNameSilva
person.familyNameOliveira Martins Tacão
person.familyNameTavares
person.familyNameMiranda
person.familyNameManaia
person.familyNameHenriques
person.givenNameIsabel
person.givenNameMarta Cristina
person.givenNameRafael D. S.
person.givenNameRita
person.givenNameCélia
person.givenNameIsabel
person.identifierC-9785-2009
person.identifier.ciencia-idB118-1C8D-84A2
person.identifier.ciencia-idE11E-AD60-B527
person.identifier.ciencia-idC31F-553B-6365
person.identifier.ciencia-idF516-BFA6-1588
person.identifier.orcid0000-0002-2994-0552
person.identifier.orcid0000-0001-7965-4928
person.identifier.orcid0000-0001-9138-5935
person.identifier.orcid0000-0003-1530-5096
person.identifier.orcid0000-0002-3674-1789
person.identifier.orcid0000-0001-7717-4939
person.identifier.scopus-author-id8691053800
person.identifier.scopus-author-id6602465318
person.identifier.scopus-author-id6701586581
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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