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Advisor(s)
Abstract(s)
Relatively little is known about the role of foods in the chain of transmission of acinetobacters and the
occurrence of different Acinetobacter spp. in foods. Currently, there is no standard procedure to recover
acinetobacters from food in order to gain insight into the food-related ecology and epidemiology of
acinetobacters. This study aimed to assess whether enrichment in Dijkshoorn enrichment medium followed
by plating in CHROMagar™ Acinetobacter medium is a useful method for the isolation of Acinetobacter
spp. from foods. Recovery of six Acinetobacter species from food spiked with these organisms
was compared for two selective enrichment media (Baumann's enrichment and Dijkshoorn's enrichment).
Significantly (p < 0.01) higher cell counts were obtained in Dijkshoorn's enrichment. Next, the
Dijkshoorn's enrichment followed by direct plating on CHROMagar™ Acinetobacter was applied to
detect Acinetobacter spp. in different foods. Fourteen different presumptive acinetobacters were recovered
and assumed to represent nine different strains on the basis of REP-PCR typing. Eight of these
strains were identified by rpoB gene analysis as belonging to the species Acinetobacter johnsonii, Acinetobacter
calcoaceticus, Acinetobacter guillouiae and Acinetobacter gandensis. It was not possible to identify
the species level of one strain which may suggests that it represents a distinct species
Description
Keywords
Acinetobacter spp. detection Enrichment Selective growth Food matrix
Citation
CARVALHEIRA, Ana; FERREIRA, Vânia; SILVA, Joana; TEIXEIRA, Paula - Enrichment of acinetobacter spp. from food samples. Food Microbiology. ISSN 0740-0020. Vol. 55 (2016), p. 123-127