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Expanding the potential of self-assembled silk fibroin as aerogel particles for tissue regeneration

dc.contributor.authorBernardes, Beatriz G.
dc.contributor.authorBaptista-Silva, Sara
dc.contributor.authorIllanes-Bordomás, Carlos
dc.contributor.authorMagalhães, Rui
dc.contributor.authorDias, Juliana Rosa
dc.contributor.authorAlves, Nuno M. F.
dc.contributor.authorCosta, Raquel
dc.contributor.authorGarcía-González, Carlos A.
dc.contributor.authorOliveira, Ana Leite
dc.date.accessioned2023-11-16T19:06:41Z
dc.date.available2023-11-16T19:06:41Z
dc.date.issued2024-09-01
dc.description.abstractA newly produced silk fibroin (SF) aerogel particulate system using a supercritical carbon dioxide (scCO2)-assisted drying technology is herein proposed for biomedical applications. Different concentrations of silk fibroin (3%, 5%, and 7% (w/v)) were explored to investigate the potential of this technology to produce size- and porosity-controlled particles. Laser diffraction, helium pycnometry, nitrogen adsorption–desorption analysis and Fourier Transform Infrared with Attenuated Total Reflectance (FTIR-ATR) spectroscopy were performed to characterize the physicochemical properties of the material. The enzymatic degradation profile of the SF aerogel particles was evaluated by immersion in protease XIV solution, and the biological properties by cell viability and cell proliferation assays. The obtained aerogel particles were mesoporous with high and concentration dependent specific surface area (203–326 m2/g). They displayed significant antioxidant activity and sustained degradation in the presence of protease XIV enzyme. The in vitro assessment using human dermal fibroblasts (HDF) confirm the particles’ biocompatibility, as well as the enhancement in cell viability and proliferation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/pharmaceutics15112605pt_PT
dc.identifier.eid85178378225
dc.identifier.issn1999-4923
dc.identifier.pmcPMC10675346
dc.identifier.pmid38004583
dc.identifier.urihttp://hdl.handle.net/10400.14/43126
dc.identifier.wos001120255400001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAerogelpt_PT
dc.subjectTissue engineeringpt_PT
dc.subjectParticles and silk fibroinpt_PT
dc.titleExpanding the potential of self-assembled silk fibroin as aerogel particles for tissue regenerationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage271
oaire.citation.issue11pt_PT
oaire.citation.issue3
oaire.citation.startPage256
oaire.citation.titlePharmaceuticspt_PT
oaire.citation.volume15pt_PT
oaire.citation.volume25
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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