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A diversity profile from the staphylococcal community on atopic dermatitis skin: a molecular approach

2013Journal article Restricted access
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dc.contributor.authorSoares, J.
dc.contributor.authorLopes, C.
dc.contributor.authorTavaria, Freni
dc.contributor.authorDelgado, L.
dc.contributor.authorPintado, M.
dc.date.accessioned2014-05-08T10:36:27Z
dc.date.available2014-05-08T10:36:27Z
dc.date.issued2013
dc.description.abstractAimsThe aim of this study was to determine the biodiversity of the skin staphylococcal community from patients with atopic dermatitis (AD) and superantigen (SAg) detection from Staphylococcus aureus isolates. Methods and ResultsIn this study, we developed a novel multiplex PCR that allows the identification and discrimination of bacteria belonging to the Staphylococcus genus both Staph.aureus and coagulase-negative Staphylococcus - Staph.capitis, Staph.epidermidis, Staph.haemolyticus and Staph.hominis isolated from the skin of patients with AD. In addition, a multiplex PCR assay that allows the rapid screening of the 19 genes that encode staphylococcal enterotoxins (SEs), SE-like toxins and toxic shock syndrome toxin-1 was also performed and applied in Staph.aureus isolates. The microflora of the skin of patients with AD was dominated by Staph.aureus (69 isolates, 356%) followed by Staph.epidermidis (59 isolates, 304%) species. The SElM and SElN genes were the most frequently detected in our study (15 isolates, 714%), followed by SEG and SElO (14 isolates, 667%). Conclusions Our molecular-based approach successfully identified the staphylococcal microflora that was relatively specific to patients with AD. Considering skin colonization and expression of virulence factors, the Staph.aureus may play a relevant role in AD pathophysiology. Significance and Impact of the StudyThis ability to classify disease-related microbial species provides new insights into the relevance of those microbes in human disorders.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationSOARES, J. ...[et al.] - A diversity profile from the staphylococcal community on atopic dermatitis skin: a molecular approach. Journal of Applied Microbiology. ISSN 1364-5072. Vol. 115 (2013), p. 1411-1419por
dc.identifier.doi10.1111/jam.12296
dc.identifier.eissn1365-2672
dc.identifier.issn1364-5072
dc.identifier.urihttp://hdl.handle.net/10400.14/14285
dc.identifier.wosWOS:000326939800015
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.subjectAtopic dermatitispor
dc.subjectGenotypingpor
dc.subjectPolymerase chain reactionpor
dc.subjectStaphylococcipor
dc.subjectSuperantigenpor
dc.titleA diversity profile from the staphylococcal community on atopic dermatitis skin: a molecular approachpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1419
oaire.citation.startPage1411
oaire.citation.titleJournal of Applied Microbiology
oaire.citation.volume115
person.familyNameOdila Pereira
person.familyNameLopes
person.familyNameKekhasharú Tavaria
person.familyNameDelgado
person.familyNamePintado
person.givenNameJoana
person.givenNameCarla
person.givenNameFreni
person.givenNameLuis
person.givenNameMaria Manuela
person.identifier2343777
person.identifierR-000-KM9
person.identifier456608
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person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0003-0300-5635
person.identifier.orcid0000-0003-1524-852X
person.identifier.orcid0000-0002-6273-6668
person.identifier.orcid0000-0003-2375-9071
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridU-9327-2017
person.identifier.ridL-8035-2013
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id57190164112
person.identifier.scopus-author-id7103222291
person.identifier.scopus-author-id6602645613
person.identifier.scopus-author-id36848414900
person.identifier.scopus-author-id7004483898
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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