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Peptide extract from spent yeast improves resistance of Saccharomyces cerevisiae to oxidative stress

2023-06-01Journal article Open access
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dc.contributor.authorLopes, Ana
dc.contributor.authorAzevedo-Silva, João
dc.contributor.authorCarsanba, Erdem
dc.contributor.authorPintado, Manuela
dc.contributor.authorOliveira, Ana Sofia
dc.contributor.authorFerreira, Carlos
dc.contributor.authorPereira, Joana Odila
dc.contributor.authorCarvalho, Ana P.
dc.contributor.authorOliveira, Carla
dc.date.accessioned2023-05-10T14:46:10Z
dc.date.available2023-05-10T14:46:10Z
dc.date.issued2023-06-01
dc.description.abstractYeast cells face various stress factors during industrial fermentations, since they are exposed to harsh environmental conditions, which may impair biomolecules productivity and yield. In this work, the use of an antioxidant peptide extract obtained from industrial spent yeast was explored as supplement for Saccharomyces cerevisiae fermentation to prevent a common bottleneck: oxidative stress. For that, a recombinant yeast strain, producer of β-farnesene, was firstly incubated with 0.5 and 0.7 g/L peptide extract, in the presence and absence of hydrogen peroxide (an oxidative stress inducer), for 1–5 h, and then assayed for intracellular reactive oxygen species, and growth ability in agar spot assays. Results showed that under 2 mM H2O2, the peptide extract could improve cells growth and reduce reactive oxygen species production. Therefore, this antioxidant effect was further evaluated in shake-flasks and 2-L bioreactor batch fermentations. Peptide extract (0.7 g/L) was able to increase yeast resistance to the oxidative stress promoted by 2 mM H2O2, by reducing reactive oxygen species levels between 1.2- and 1.7-fold in bioreactor and between 1.2- and 3-fold in shake-flask fermentations. Moreover, improvements on yeast cell density of up to 1.5-fold and 2-fold, and on biomolecule concentration of up to 1.6-fold and 2.8-fold, in bioreactor and shake-flasks, respectively, were obtained. Thus, culture medium supplementation with antioxidant peptide extracted from industrial spent yeast is a promising strategy to improve fermentation performance while valuing biomass waste. This valorization can promote a sustainable and eco-friendly solution for the biotechnology industry by the implementation of a circular economy model.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00253-023-12514-3pt_PT
dc.identifier.eid85153218217
dc.identifier.eidPMC10175367
dc.identifier.issn0175-7598
dc.identifier.pmid37086282
dc.identifier.urihttp://hdl.handle.net/10400.14/41086
dc.identifier.wos000973374700001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationPOCI-01–0247-FEDER-027578
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectBioreactor fermentationpt_PT
dc.subjectCircular economypt_PT
dc.subjectFarnesene productionpt_PT
dc.subjectPeptide-rich extractpt_PT
dc.subjectReactive oxygen speciespt_PT
dc.titlePeptide extract from spent yeast improves resistance of Saccharomyces cerevisiae to oxidative stresspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3417
oaire.citation.issue11
oaire.citation.startPage3405
oaire.citation.titleApplied Microbiology and Biotechnologypt_PT
oaire.citation.volume107
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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