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Incorporation of beads into oral films for buccal and oral delivery of bioactive molecules

2018Journal article Open access
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dc.contributor.authorCastro, Pedro M.
dc.contributor.authorSousa, Flávia
dc.contributor.authorMagalhães, Rui
dc.contributor.authorRuiz-Henestrosaf, Victor Manuel Pizones
dc.contributor.authorPilosoff, Ana M.R.
dc.contributor.authorMadureira, Ana Raquel
dc.contributor.authorSarmento, Bruno
dc.contributor.authorPintado, Manuela E
dc.date.accessioned2018-09-17T16:14:13Z
dc.date.available2018-09-17T16:14:13Z
dc.date.issued2018
dc.description.abstractThe association of alginate beads and guar-gum films in a single delivery system was idealized to promote a more effective buccal and oral delivery of bioactive molecules. A response surface method (experimental design approach) was performed to obtain optimal formulations of alginate beads to be incorporated into guar gum oral films as combined buccal and oral delivery systems for caffeine delivery. The combined formulation was further characterized regarding physicochemical properties, drug release, cell viability and buccal permeability. Beads average size, determined by dynamic light scattering (DLS), was of 3.37 ± 6.36 μm. Film thickness was set to 62 μm. Scanning electron microscopy micrographs revealed that beads were evenly distributed onto the film matrix and beads size was in accordance to data obtained from DLS analysis. Evaluation of Fourier-transform infrared spectra did not indicate the formation of new covalent bonds between the matrix of guar-gum films, alginate beads and caffeine. In vitro release assays by dialysis membrane allowed understanding that the combination of guar-gum films and alginate beads assure a slower release of caffeine when compared with the delivery profile of free caffeine from alginate beads or guar-gum films alone. MTT assay, performed on human buccal carcinoma TR146 cell line, allowed concluding that neither guar-gum film, alginate beads nor guar-gum film incorporated into alginate beads significantly compromised cell viability after 12 h of exposure. As demonstrated by in vitro permeability assay using TR146 human buccal carcinoma cell lines, combination of guargum films and alginate beads also promoted a slower release and, thus, lower apparent permeability (1.15E–05 ± 3.50E-06) than for caffeine solution (2.68E–05 ± 7.30E-06), guar-gum film (3.12E–05 ± 4.70E-06) or alginate beads (2.01E–05 ± 3.90E-06). The conjugation of alginate beads within an orodispersible film matrix represents an effective oral/buccal delivery system that induces a controlled release along with an enhanced intimate contact with cell layers that may promote higher in vivo bioavailability of carried drugs.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.citationCastro, P.M., Sousa, F., Magalhães, R., Ruiz-Henestrosa, V.M.P, Pilosof, A. M.R., Madureira, A. R., … Pintado, M.E. (2018). Incorporation of beads into oral films for buccal and oral delivery of bioactive molecules. Carbohydrate Polymers, 194, 411–421pt_PT
dc.identifier.doi10.1016/j.carbpol.2018.04.032pt_PT
dc.identifier.eid85046802639
dc.identifier.eissn1879-1344
dc.identifier.issn0144-8617
dc.identifier.pmid29801856
dc.identifier.urihttp://hdl.handle.net/10400.14/25696
dc.identifier.wos000433134900049
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationNORTE-01-0145-FEDER000012pt_PT
dc.relationNORTE-08-5369-FSE-000007pt_PT
dc.relationStudy of biophysical interactions and antibody bioactivity of bevacizumab encapsulated into nanoparticles towards its controlled release
dc.relationDevelopment of oral strips containing nanoencapsulated bioactive compounds
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0
dc.subjectAlginate beadspt_PT
dc.subjectCaffeinept_PT
dc.subjectDrug deliverypt_PT
dc.subjectExperimental designpt_PT
dc.subjectOral filmspt_PT
dc.subjectSlow releasept_PT
dc.titleIncorporation of beads into oral films for buccal and oral delivery of bioactive moleculespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStudy of biophysical interactions and antibody bioactivity of bevacizumab encapsulated into nanoparticles towards its controlled release
oaire.awardTitleDevelopment of oral strips containing nanoencapsulated bioactive compounds
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F112201%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBBB-NAN%2F3249%2F2014/PT
oaire.citation.endPage421
oaire.citation.startPage411
oaire.citation.titleCarbohydrate Polymerspt_PT
oaire.citation.volume194
oaire.fundingStream3599-PPCDT
person.familyNameMiranda de Castro
person.familyNameSousa
person.familyNameMagalhães
person.familyNameMadureira
person.familyNameSarmento
person.familyNamePintado
person.givenNamePedro João
person.givenNameFlávia
person.givenNameRui
person.givenNameRaquel
person.givenNameBruno
person.givenNameMaria Manuela
person.identifierK-1359-2014
person.identifierM-4659-2013
person.identifier152609
person.identifier456608
person.identifier.ciencia-id5112-6B99-DC1F
person.identifier.ciencia-idAF15-BA82-CFA6
person.identifier.ciencia-id4C1A-BDD4-D716
person.identifier.ciencia-id1C14-25F8-CC07
person.identifier.ciencia-idE816-B6B0-06A5
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0001-7164-8005
person.identifier.orcid0000-0001-6587-6175
person.identifier.orcid0000-0003-3172-440X
person.identifier.orcid0000-0001-6596-1927
person.identifier.orcid0000-0001-5763-7553
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridK-1359-2014
person.identifier.ridM-4659-2013
person.identifier.ridJ-6265-2013
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id57201808340
person.identifier.scopus-author-id56385224800
person.identifier.scopus-author-id6603813414
person.identifier.scopus-author-id8384097900
person.identifier.scopus-author-id57194334236
person.identifier.scopus-author-id7004483898
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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