Logo do repositório
 
Publicação

Establishing a dengue genomic monitoring in Cuba: uncovering virus dynamics to enhance local response

2025-09-01Artigo de investigação Acesso aberto
Ver registo simples
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorPérez, Melissa
dc.contributor.authorÁlvarez, Mayling
dc.contributor.authorPerez, Lissette
dc.contributor.authorBenitez, Ana Julia
dc.contributor.authorSerrano, Silvia
dc.contributor.authorAdelino, Talita Emile Ribeiro
dc.contributor.authorFonseca, Vagner
dc.contributor.authorResik, Sonia
dc.contributor.authorKouri, Vivian
dc.contributor.authorGresh, Lionel
dc.contributor.authorFranco, Leticia
dc.contributor.authorRico, Jairo Mendez
dc.contributor.authorLourenço, José
dc.contributor.authorAlcantara, Luiz Carlos Junior
dc.contributor.authorGuzman, Maria G.
dc.contributor.authorGiovanetti, Marta
dc.date.accessioned2025-09-02T13:44:49Z
dc.date.available2025-09-02T13:44:49Z
dc.date.issued2025-09-01
dc.description.abstractBackground: A major dengue outbreak was reported by the Cuban Ministry of Health in 2022, highlighting the need for strong surveillance, with high pediatric cases showing warning signs. Using phylogenetic and epidemiologic approaches, we provide evidence toward a better understanding of dengue's recent transmission history. Methods: This study details the implementation of the genomic surveillance system for Dengue virus. Pre-screened DENV-3 positive samples (reverse transcription–polymerase chain reaction [RT-PCR] cycle threshold [Ct] <30) with epidemiologic metadata—including symptom onset, sample collection date, sex, age, residence, symptoms, and disease classification—underwent whole genome sequencing using Nanopore technology. Results: Genome sequences from 2022 to 2023 confirmed DENV-3 genotype III circulation in western, central, and eastern Cuba, covering 15 provinces and one special municipality. All cases were autochthonous, with 93% classified as dengue without warning signs and 7% as severe dengue. Long-term analysis identified a seasonal window of heightened suitability from May to November. Phylogenetic analyses revealed that DENV-3 genotype III has been introduced into Cuba multiple times, primarily from Asia and the Americas. These independent introduction events, likely driven by international travel and trade, underscore the importance of continued viral importation monitoring. The newly sequenced Cuban DENV-3 genotype III genomes were assigned to two distinct sub-lineages within genotype III: 3III_C, corresponding to the older American lineage I (2001-2014), and 3III_B.3, representing a novel introduction into Cuba associated with the more recent American lineage II (2022-2023). Evidence suggests that 3III_C, previously circulating in the country, may have been replaced by 3III_B.3 around 2020. Conclusions: This study outlines the development of a dengue genomic monitoring system in the country. The system will contribute to Cuba's public health response, facilitating timely interventions and potentially reducing the disease's impact on the local population.eng
dc.identifier.citationPérez, M., Álvarez, M., Perez, L., & Benitez, A. J. et al. (2025). Establishing a dengue genomic monitoring in Cuba: uncovering virus dynamics to enhance local response. IJID Regions, 16, Article 100683. https://doi.org/10.1016/j.ijregi.2025.100683
dc.identifier.doi10.1016/j.ijregi.2025.100683
dc.identifier.eid105009981222
dc.identifier.issn2772-7076
dc.identifier.other64013cb4-6853-41c2-b119-39151bc0e772
dc.identifier.pmcPMC12257021
dc.identifier.pmid40662128
dc.identifier.urihttp://hdl.handle.net/10400.14/54607
dc.identifier.wos001533860400001
dc.language.isoeng
dc.peerreviewedyes
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCaribbean
dc.subjectCuba
dc.subjectDengue virus
dc.subjectEco-epidemiologic modelling
dc.subjectGenomic monitoring
dc.titleEstablishing a dengue genomic monitoring in Cuba: uncovering virus dynamics to enhance local responseeng
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.titleIJID Regions
oaire.citation.volume16
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
124432296.pdf
Tamanho:
1.64 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

CBR - Contribuições em Revistas Científicas / Contribution to Journals