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Advisor(s)
Abstract(s)
The aim of this research effort was to investigate the role of various sugar substrates
in the growth medium upon thermotolerance and upon survival during storage after
freeze-drying of Lactobacillus bulgaricus. Addition of the sugars tested to the growth
medium, and of these and sorbitol to the drying medium (skim milk) was investigated
so as to determine whether a relationship exists between growth and drying media,
in terms of protection of freeze-dried cells throughout storage. The lowest decrease in
viability of L. bulgaricus cells after freeze-drying was obtained when that organism
was grown in the presence of mannose. However, L. bulgaricus clearly survived better
during storage when cells had been grown in the presence of fructose, lactose or
mannose rather than glucose (the standard sugar in the growth medium). A similar
effect could not be observed in terms of thermotolerance; in this case, the growth
medium supplemented with lactose was found to yield cells bearing the highest heat
resistance. Supplementation of the drying medium with glucose, fructose, lactose,
mannose or sorbitol led in most cases to enhancement of protection during storage, to
a degree that was growth medium-dependent.
Description
Keywords
Pedagogical Context
Citation
"Biotechnology progress" . ISSN 8756-7938. 20:2 (2004) 248-254
Publisher
American Chemical Society and American Institute of Chemical Engineers