Publication
Biodegradation and Metabolic Pathway of 17 beta-Estradiol by Rhodococcus sp. ED55
dc.contributor.author | Moreira, Irina S. | |
dc.contributor.author | Murgolo, Sapia | |
dc.contributor.author | Mascolo, Giuseppe | |
dc.contributor.author | Castro, Paula M. L. | |
dc.date.accessioned | 2022-06-21T09:21:49Z | |
dc.date.available | 2022-06-21T09:21:49Z | |
dc.date.issued | 2022-05-30 | |
dc.description.abstract | Endocrine disrupting compounds (EDCs) in the environment are considered a motif of concern, due to the widespread occurrence and potential adverse ecological and human health effects. The natural estrogen, 17 beta-estradiol (E2), is frequently detected in receiving water bodies after not being efficiently removed in conventional wastewater treatment plants (WWTPs), promoting a negative impact for both the aquatic ecosystem and human health. In this study, the biodegradation of E2 by Rhodococcus sp. ED55, a bacterial strain isolated from sediments of a discharge point of WWTP in Coloane, Macau, was investigated. Rhodococcus sp. ED55 was able to completely degrade 5 mg/L of E2 in 4 h in a synthetic medium. A similar degradation pattern was observed when the bacterial strain was used in wastewater collected from a WWTP, where a significant improvement in the degradation of the compound occurred. The detection and identification of 17 metabolites was achieved by means of UPLC/ESI/HRMS, which proposed a degradation pathway of E2. The acute test with luminescent marine bacterium Aliivibrio fischeri revealed the elimination of the toxicity of the treated effluent and the standardized yeast estrogenic (S-YES) assay with the recombinant strain of Saccharomyces cerevisiae revealed a decrease in the estrogenic activity of wastewater samples after biodegradation. | pt_PT |
dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
dc.identifier.doi | 10.3390/ijms23116181 | pt_PT |
dc.identifier.eid | 85131705122 | |
dc.identifier.issn | 1661-6596 | |
dc.identifier.pmc | PMC9181579 | |
dc.identifier.pmid | 35682859 | |
dc.identifier.uri | http://hdl.handle.net/10400.14/37901 | |
dc.identifier.wos | 000808863900001 | |
dc.language.iso | eng | pt_PT |
dc.peerreviewed | yes | pt_PT |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
dc.subject | Endocrine disrupting chemicals | pt_PT |
dc.subject | Rhodococcus sp. ED55 | pt_PT |
dc.subject | Bioaugmentation | pt_PT |
dc.subject | Wastewater | pt_PT |
dc.subject | 17 beta-estradiol | pt_PT |
dc.title | Biodegradation and Metabolic Pathway of 17 beta-Estradiol by Rhodococcus sp. ED55 | pt_PT |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.citation.issue | 11 | pt_PT |
oaire.citation.title | International Journal of Molecular Sciences | pt_PT |
oaire.citation.volume | 23 | pt_PT |
rcaap.rights | openAccess | pt_PT |
rcaap.type | article | pt_PT |
Files
Original bundle
1 - 1 of 1