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Biodegradation and Metabolic Pathway of 17 beta-Estradiol by Rhodococcus sp. ED55

2022-05-30Journal article Open access
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dc.contributor.authorMoreira, Irina S.
dc.contributor.authorMurgolo, Sapia
dc.contributor.authorMascolo, Giuseppe
dc.contributor.authorCastro, Paula M. L.
dc.date.accessioned2022-06-21T09:21:49Z
dc.date.available2022-06-21T09:21:49Z
dc.date.issued2022-05-30
dc.description.abstractEndocrine disrupting compounds (EDCs) in the environment are considered a motif of concern, due to the widespread occurrence and potential adverse ecological and human health effects. The natural estrogen, 17 beta-estradiol (E2), is frequently detected in receiving water bodies after not being efficiently removed in conventional wastewater treatment plants (WWTPs), promoting a negative impact for both the aquatic ecosystem and human health. In this study, the biodegradation of E2 by Rhodococcus sp. ED55, a bacterial strain isolated from sediments of a discharge point of WWTP in Coloane, Macau, was investigated. Rhodococcus sp. ED55 was able to completely degrade 5 mg/L of E2 in 4 h in a synthetic medium. A similar degradation pattern was observed when the bacterial strain was used in wastewater collected from a WWTP, where a significant improvement in the degradation of the compound occurred. The detection and identification of 17 metabolites was achieved by means of UPLC/ESI/HRMS, which proposed a degradation pathway of E2. The acute test with luminescent marine bacterium Aliivibrio fischeri revealed the elimination of the toxicity of the treated effluent and the standardized yeast estrogenic (S-YES) assay with the recombinant strain of Saccharomyces cerevisiae revealed a decrease in the estrogenic activity of wastewater samples after biodegradation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/ijms23116181pt_PT
dc.identifier.eid85131705122
dc.identifier.issn1661-6596
dc.identifier.pmcPMC9181579
dc.identifier.pmid35682859
dc.identifier.urihttp://hdl.handle.net/10400.14/37901
dc.identifier.wos000808863900001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEndocrine disrupting chemicalspt_PT
dc.subjectRhodococcus sp. ED55pt_PT
dc.subjectBioaugmentationpt_PT
dc.subjectWastewaterpt_PT
dc.subject17 beta-estradiolpt_PT
dc.titleBiodegradation and Metabolic Pathway of 17 beta-Estradiol by Rhodococcus sp. ED55pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue11pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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