Repository logo
 
Publication

Spray-drying encapsulation of the live biotherapeutic candidate Akkermansia muciniphila DSM 22959 to survive aerobic storage

2022-05Journal article Open access
Simple item page
dc.contributor.authorBarbosa, Joana Cristina
dc.contributor.authorAlmeida, Diana
dc.contributor.authorMachado, Daniela
dc.contributor.authorSousa, Sérgio
dc.contributor.authorFreitas, Ana Cristina
dc.contributor.authorAndrade, José Carlos
dc.contributor.authorGomes, Ana Maria
dc.date.accessioned2022-05-20T15:44:21Z
dc.date.available2022-05-20T15:44:21Z
dc.date.issued2022-05
dc.description.abstractAkkermansia muciniphila is regarded as a promising next-generation probiotic or live biotherapeutic candidate. Effective delivery strategies must be developed to ensure high enough viability of the probiotic strain throughout its industrial formulation, distribution chain, shelf-life, and, ultimately, the host’s gastrointestinal tract, where it should exert its beneficial effect(s). Among the possible methodologies, spray-drying is considered industrially attractive regarding its costs, efficiency, and scalability, with the due parameter customization. In this study, spray-drying was explored as a one-step process to encapsulate A. muciniphila DSM 22959, testing the drying settings and three different dairy-based matrices. Microcapsule morphology and size was assessed, and viability throughout storage at 4 or 22 °C and simulated gastrointestinal passage was determined. Akkermansia muciniphila microencapsulation by spray-drying, using 10% skim milk and inlet/outlet temperatures of 150/65 °C, is effective in terms of viability stabilization, both during prolonged aerobic storage and exposure to simulated gastrointestinal passage. Akkermansia muciniphila viability was maintained at around 107 CFU/g up to 28 days at 4 °C under aerobic conditions with viability losses inferior to 1 log reduction. This methodology provides the necessary conditions to efficiently deliver the recommended dose of live A. muciniphila in the human gut as a live biotherapeutic product.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/ph15050628pt_PT
dc.identifier.eid85130559245
dc.identifier.issn1424-8247
dc.identifier.pmcPMC9143277
dc.identifier.pmid35631454
dc.identifier.urihttp://hdl.handle.net/10400.14/37680
dc.identifier.wos000802372700001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAerobic storagept_PT
dc.subjectAkkermansia muciniphilapt_PT
dc.subjectMicroencapsulationpt_PT
dc.subjectSimulated gastrointestinal passagept_PT
dc.subjectSpray-dryingpt_PT
dc.subjectStabilitypt_PT
dc.titleSpray-drying encapsulation of the live biotherapeutic candidate Akkermansia muciniphila DSM 22959 to survive aerobic storagept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F00520%2F2017%2FCP1404%2FCT0001/PT
oaire.citation.endPage13pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePharmaceuticalspt_PT
oaire.citation.volume15pt_PT
oaire.fundingStreamCEEC IND 2017
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication077fbbcc-1b7e-49e5-a895-ad5c9f4fc572
relation.isProjectOfPublication.latestForDiscovery077fbbcc-1b7e-49e5-a895-ad5c9f4fc572

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
43746045.pdf
Size:
1.81 MB
Format:
Adobe Portable Document Format
Download

Collections

CBQF - Contribuições em Revistas Científicas / Contribution to Journals