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Genetic variation in the conjugative plasmidome of a hospital effluent multidrug resistant Escherichia coli strain

2019Journal article Restricted access
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dc.contributor.authorFerreira, Catarina
dc.contributor.authorBogas, Diana
dc.contributor.authorBikarolla, Santosh K.
dc.contributor.authorVarela, Ana Rita
dc.contributor.authorFrykholm, Karolin
dc.contributor.authorLinheiro, Raquel
dc.contributor.authorNunes, Olga C.
dc.contributor.authorWesterlund, Fredrik
dc.contributor.authorManaia, Célia M.
dc.date.accessioned2019-01-07T12:48:36Z
dc.date.available2019-01-07T12:48:36Z
dc.date.issued2019
dc.description.abstractBacteria harboring conjugative plasmids have the potential for spreading antibiotic resistance through horizontal gene transfer. It is described that the selection and dissemination of antibiotic resistance is enhanced by stressors, like metals or antibiotics, which can occur as environmental contaminants. This study aimed at unveiling the composition of the conjugative plasmidome of a hospital effluent multidrug resistant Escherichia coli strain (H1FC54) under different mating conditions. To meet this objective, plasmid pulsed field gel electrophoresis, optical mapping analyses and DNA sequencing were used in combination with phenotype analysis. Strain H1FC54 was observed to harbor five plasmids, three of which were conjugative and two of these, pH1FC54_330 and pH1FC54_140, contained metal and antibiotic resistance genes. Transconjugants obtained in the absence or presence of tellurite (0.5 μM or 5 μM), arsenite (0.5 μM, 5 μM or 15 μM) or ceftazidime (10 mg/L) and selected in the presence of sodium azide (100 mg/L) and tetracycline (16 mg/L) presented distinct phenotypes, associated with the acquisition of different plasmid combinations, including two co-integrate plasmids, of 310 kbp and 517 kbp. The variable composition of the conjugative plasmidome, the formation of co-integrates during conjugation, as well as the transfer of non-transferable plasmids via co-integration, and the possible association between antibiotic, arsenite and tellurite tolerance was demonstrated. These evidences bring interesting insights into the comprehension of the molecular and physiological mechanisms that underlie antibiotic resistance propagation in the environment.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFerreira, C., Bogas, D., Bikarolla, S. K., Varela, A. R., Frykholm, K., Linheiro, R., … Manaia, C. M. (2019). Genetic variation in the conjugative plasmidome of a hospital effluent multidrug resistant Escherichia coli strain. Chemosphere, 220, 748–759. https://doi.org/10.1016/j.chemosphere.2018.12.130pt_PT
dc.identifier.doi10.1016/j.chemosphere.2018.12.130pt_PT
dc.identifier.eid85059329975
dc.identifier.eissn1879-1298
dc.identifier.issn0045-6535
dc.identifier.pmid30611073
dc.identifier.urihttp://hdl.handle.net/10400.14/26620
dc.identifier.wos000458591200083
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPOCI-01-0145-FEDER-006939
dc.subjectArsenitept_PT
dc.subjectOptical DNA mappingpt_PT
dc.subjectPFGEpt_PT
dc.subjectPacBiopt_PT
dc.subjectTelluritept_PT
dc.titleGenetic variation in the conjugative plasmidome of a hospital effluent multidrug resistant Escherichia coli strainpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/ENMed%2F0001%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FEQU%2F00511%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.citation.endPage759pt_PT
oaire.citation.startPage748pt_PT
oaire.citation.titleChemospherept_PT
oaire.citation.volume220
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameBogas
person.familyNameVarela
person.familyNameLinheiro
person.familyNameNunes
person.familyNameWesterlund
person.familyNameManaia
person.givenNameDiana
person.givenNameAna Rita
person.givenNameRaquel
person.givenNameOlga
person.givenNameFredrik
person.givenNameCélia
person.identifier.ciencia-idDA19-1BA2-4088
person.identifier.ciencia-idBA14-BD30-C9AF
person.identifier.ciencia-id951D-5065-FFCB
person.identifier.ciencia-idC31F-553B-6365
person.identifier.orcid0000-0002-1037-5784
person.identifier.orcid0000-0001-9691-0217
person.identifier.orcid0000-0003-2659-0910
person.identifier.orcid0000-0003-4742-2537
person.identifier.orcid0000-0002-4767-4868
person.identifier.orcid0000-0002-3674-1789
person.identifier.ridA-2340-2011
person.identifier.scopus-author-id57194574241
person.identifier.scopus-author-id7005088596
person.identifier.scopus-author-id6505916424
person.identifier.scopus-author-id6602465318
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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