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Transient receptor potential vanilloid 1 is expressed in human cytotrophoblasts: Induction of cell apoptosis and impairment of syncytialization

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dc.contributor.authorCosta, M. A.
dc.contributor.authorFonseca, B. M.
dc.contributor.authorKeating, E.
dc.contributor.authorTeixeira, N. A.
dc.contributor.authorCorreia-da-Silva, G.
dc.date.accessioned2015-05-11T16:52:37Z
dc.date.available2015-05-11T16:52:37Z
dc.date.issued2014
dc.description.abstractThe normal development of placenta relies essentially on a balanced proliferation, differentiation andapoptosis of cytotrophoblasts. These processes are tightly regulated by several hormones, cytokines,lipids and other molecules and anomalies in these events are associated with gestational complications.The cation channel transient receptor potential vanilloid 1 (TRPV1) is expressed in several organs andtissues and it participates in cellular events like nociception, inflammation and cell death. However, theexpression and importance of this receptor in human placenta still remains unknown. In this work, wefound that TRPV1 is expressed in human cytotrophoblasts and syncytiotrophoblasts. Furthermore, theTRPV1 agonists capsaicin and anandamide decreased cytotrophoblast viability and induced morpho-logical alterations, such as chromatin condensation and fragmentation, which suggest the occurrenceof apoptosis. Also, both TRPV1 agonists induced a loss of mitochondrial membrane potential and anincrease of caspase 3/7 activity and production of reactive species of oxygen and nitrogen. Furthermore,capsaicin (10 M) impaired the spontaneous in vitro differentiation of cytotrophoblasts into syncytiotro-phoblasts by triggering TRPV1, as observed by the decrease in placental alkaline phosphatase activity andin human chorionic gonadotropin secretion. On the other hand, anandamide decreased placental alkalinephosphatase activity via a TRPV1-independent mechanism but did not influence the secretion of humanchorionic gonadotropin. In conclusion, we showed that TRPV1 is expressed in human cytotrophoblastsand syncytiotrophoblasts and also reported the involvement of this receptor in cytotrophoblast apoptosisand differentiation.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationCOSTA, M. A… [et al.] - Transient receptor potential vanilloid 1 is expressed in human cytotrophoblasts: Induction of cell apoptosis and impairment of syncytialization. The International Journal of Biochemistry & Cell Biology. ISSN 1357-2725. Vol. 57 (2014), p. 177-85por
dc.identifier.doi10.1016/j.biocel.2014.10.008
dc.identifier.issn1357-2725
dc.identifier.urihttp://hdl.handle.net/10400.14/17545
dc.identifier.wosWOS:000347662300021
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevier
dc.relationENDOCANNABINOID SYSTEM AND PLACENTAL DEVELOPMENT: IMPORTANCE IN TROPHOBLAST CELLULAR TURNOVER AND MODULATION OF PROTEIN EXPRESSION
dc.relationENDOCANNABINOID SIGNALLING SYSTEM AND ITS INFLUENCE IN MATERNAL TISSUES REMODELLING: IMPLICATIONS TO PREGNANCY OUTCOME
dc.subjectAnandamidepor
dc.subjectApoptosispor
dc.subjectCapsaicinpor
dc.subjectCytotrophoblastspor
dc.subjectSyncytializationpor
dc.subjectTRPV1por
dc.titleTransient receptor potential vanilloid 1 is expressed in human cytotrophoblasts: Induction of cell apoptosis and impairment of syncytializationpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleENDOCANNABINOID SYSTEM AND PLACENTAL DEVELOPMENT: IMPORTANCE IN TROPHOBLAST CELLULAR TURNOVER AND MODULATION OF PROTEIN EXPRESSION
oaire.awardTitleENDOCANNABINOID SIGNALLING SYSTEM AND ITS INFLUENCE IN MATERNAL TISSUES REMODELLING: IMPLICATIONS TO PREGNANCY OUTCOME
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F70721%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F72958%2F2010/PT
oaire.citation.endPage185
oaire.citation.startPage177
oaire.citation.titleThe International Journal of Biochemistry & Cell Biology
oaire.citation.volume57
person.familyNameCosta
person.familyNameFonseca
person.familyNameKeating
person.familyNameTeixeira
person.familyNameCorreia-da-Silva
person.givenNameMariana
person.givenNameBruno M
person.givenNameElisa
person.givenNameNatercia
person.givenNameGeorgina
person.identifier517090
person.identifier.ciencia-id2D1C-219E-C087
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person.identifier.ciencia-id271B-EF0A-828D
person.identifier.orcid0000-0002-5798-0236
person.identifier.orcid0000-0002-8873-5591
person.identifier.orcid0000-0002-3904-9907
person.identifier.orcid0000-0002-3664-1120
person.identifier.orcid0000-0001-5787-9018
person.identifier.ridL-6318-2014
person.identifier.ridE-9036-2011
person.identifier.ridK-4663-2013
person.identifier.ridK-4696-2013
person.identifier.scopus-author-id56357778400
person.identifier.scopus-author-id35169100300
person.identifier.scopus-author-id35612071300
person.identifier.scopus-author-id7006354794
person.identifier.scopus-author-id35616148100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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