Publication
Influence of the coagulant level on early proteolysis in ovine cheese-like systems made with sterilized milk and Cynara cardunculus
| dc.contributor.author | Silva, S. V. | |
| dc.contributor.author | Malcata, F. X. | |
| dc.date.accessioned | 2011-10-21T15:28:55Z | |
| dc.date.available | 2011-10-21T15:28:55Z | |
| dc.date.issued | 2004-08 | |
| dc.description.abstract | The effect of coagulant level on the quality and quantity of protein breakdown during the first 24 h of ripening of cheese-like systems, manufactured with sterilized ovine milk using crude aqueous extracts of Cynara cardunculus as coagulant, was experimentally assessed. Urea-polyacrylamide gel electrophoresis was performed on both water-soluble and water-insoluble cheese extracts to monitor the casein degradation pattern; the ripening extension index and the ripening depth index were thus calculated. Peptides from the water-soluble fraction were isolated by reverse-phase, high-performance liquid chromatography and partially sequenced by Edman degradation. Higher residual coagulant levels in curdled milk led to earlier breakdown of caseins, as expected. The primary cleavage sites were Phe105-Met106 in k-casein, Phe23-Val24 in s1-casein, and Leu127-Thr128, Ser142-Trp143, Leu165-Ser166, and Leu190-Tyr191 in β-casein. | por |
| dc.identifier.citation | "Journal of Food Science". ISSN 0022-1147. 69:7 (2004) C579-C584 | por |
| dc.identifier.uri | http://hdl.handle.net/10400.14/6719 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Wiley | por |
| dc.relation.publisherversion | The definitive version is available at www3.interscience.wiley.com | por |
| dc.subject | Plant rennet | por |
| dc.subject | Ripening | por |
| dc.subject | Dairy food | por |
| dc.subject | HPLC | por |
| dc.subject | Electrophoresis | por |
| dc.title | Influence of the coagulant level on early proteolysis in ovine cheese-like systems made with sterilized milk and Cynara cardunculus | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| person.familyName | Malcata | |
| person.givenName | Francisco | |
| person.identifier.ciencia-id | 1B13-38A5-35F5 | |
| person.identifier.orcid | 0000-0003-3073-1659 | |
| person.identifier.scopus-author-id | 7102542478 | |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | a06c00da-0e2f-4434-8ede-4d7b22c0dfe9 | |
| relation.isAuthorOfPublication.latestForDiscovery | a06c00da-0e2f-4434-8ede-4d7b22c0dfe9 |
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