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Fish by-products: a source of enzymes to generate circular bioactive hydrolysates

2023-01-24Journal article Open access
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dc.contributor.authorBorges, Sandra
dc.contributor.authorOdila, Joana
dc.contributor.authorVoss, Glenise
dc.contributor.authorMartins, Rui
dc.contributor.authorRosa, Ana
dc.contributor.authorCouto, José António
dc.contributor.authorAlmeida, André
dc.contributor.authorPintado, Manuela
dc.date.accessioned2023-02-07T11:08:47Z
dc.date.available2023-02-07T11:08:47Z
dc.date.issued2023-01-24
dc.description.abstractFish viscera are usually discarded as waste, causing environmental problems, or as low-value by-products. This study describes a self-sufficient and zero waste approach to obtain enzymes and protein hydrolysates from fish by-products. Firstly, recovery steps of viscera enzymatic extract were applied, and the resulting raw extract was stable at a pH range of 8–9 and at temperatures between 40 and 50 °C. The application of the extracted enzymes and alcalase on fish by-products hydrolysis was also determined. The selected conditions for the enzymatic hydrolysis were 10% (E/S) for 6 h using viscera enzymatic extract and 3% (E/S) for 2 h using alcalase. Fish protein hydrolysates (FPH) proved to have a notable antioxidant capacity with similar activity, ~11 mg ascorbic acid/g dry extract (ABTS assay) and ~150 mg Trolox/g dry extract (ORAC assay). FPH were also able to inhibit the angiotensin-converting enzyme, however, alcalase hydrolysates revealed a higher antihypertensive potential, IC50 of 101 µg of protein/mL. In general, FPH obtained by both enzymes systems maintained these bioactivities after the passage throughout a simulated gastrointestinal tract. The hydrolysates also displayed important technological properties, namely oil absorption capacity (~1 g oil/g sample) and emulsifying property (~40%). Therefore, it will be conceivable to use fish by-products based on a circular economy approach to generate added value compounds for animal and human nutritionpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/molecules28031155pt_PT
dc.identifier.eid85147893374
dc.identifier.issn1420-3049
dc.identifier.pmcPMC9919145
dc.identifier.pmid36770822
dc.identifier.urihttp://hdl.handle.net/10400.14/40135
dc.identifier.wos000935807500001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectViscerapt_PT
dc.subjectProtein hydrolysispt_PT
dc.subjectBioactive peptidespt_PT
dc.subjectTechnological propertiespt_PT
dc.titleFish by-products: a source of enzymes to generate circular bioactive hydrolysatespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue3pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume28pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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