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Polygalacturonase-mediated dissolution and depolymerization of pectins in solutions mimicking the pH and mineral composition of tomato fruit apoplast

2007Journal article Open access
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dc.contributor.authorAlmeida, Domingos P. F.
dc.contributor.authorHuber, Donald J.
dc.date.accessioned2010-10-11T21:39:00Z
dc.date.available2010-10-11T21:39:00Z
dc.date.issued2007
dc.description.abstractThe effects of polygalacturonase (PG) on pectin dissolution and depolymerization were examined in cell walls from mature-green tomato fruit incubated in a conventional (C) buffer (30 mMsodium acetate, 150 mMNaCl, pH 4.5) and in buffers mimicking the apoplastic solution of maturegreen (MG) and ripe fruit (R). Pectin dissolution from cell walls was much higher in C-buffer than in MG- or R-buffers. Buffered phenol inactivated cell walls incubated in C-buffer released 4.9 mg mg 1 pectin, which increased to 86.4 mg mg 1 when PG was added. In the R-buffer, PG increased the pectin dissolution from inactive cell walls from 0.5 to 18.3 mg mg 1. However, when the assay was conducted in buffer mimicking maturegreen fruits, added PG did not increase pectin dissolution. The release of uronic acids from active cell walls in C-buffer and R-buffer was consistently lower than that from inactive walls due to the activity of pectinmethylesterase. Gel filtration profiles of CDTA-soluble pectins extracted from cell walls previously incubated in C-buffer or R-buffer with PG reveal that the enzyme is capable of hydrolyzing insoluble, ionically bound, pectins. These data support the idea that pH and mineral composition of the fruit apoplast provide a means for biochemical regulation of cell wall metabolism.por
dc.identifier.citation"Plant science". ISSN 0168-9452. 172: 6 (2007) 1087-1094por
dc.identifier.urihttp://hdl.handle.net/10400.14/2842
dc.language.isoengpor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TBH-4NBBYKR-1&_user=10&_coverDate=06%2F30%2F2007&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_searchStrId=1493817930&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=a8e321dca46300fb4482fb4bc08bb3ac&searchtype=apor
dc.subjectCalciumpor
dc.subjectCell wallpor
dc.subjectLycopersicon esculentumpor
dc.subjectPectinmethylesterasepor
dc.subjectPolyuronidespor
dc.subjectPolygalacturonasepor
dc.titlePolygalacturonase-mediated dissolution and depolymerization of pectins in solutions mimicking the pH and mineral composition of tomato fruit apoplastpor
dc.typejournal article
dspace.entity.typePublication
rcaap.rightsopenAccesspor
rcaap.typearticlepor

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