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Enzymatic hydrolysis allows an integral valorization of nannochloropsis oceanica resulting in the production of bioactive peptide extracts and an eicosapentaenoic acid enriched fraction

dc.contributor.authorCunha, Sara A.
dc.contributor.authorCoscueta, Ezequiel R.
dc.contributor.authorAlexandre, Agostinho M. R. C.
dc.contributor.authorPartidário, Ana Maria Carvalho
dc.contributor.authorFernández, Naiara
dc.contributor.authorPaiva, Alexandre
dc.contributor.authorSilva, Joana Laranjeira
dc.contributor.authorPintado, Manuela E.
dc.date.accessioned2024-02-29T11:35:24Z
dc.date.available2024-11-27T01:31:08Z
dc.date.issued2024-02
dc.description.abstractNannochloropsis oceanica is a microalga with relevant protein content, making it a potential source of bioactive peptides. Furthermore, it is also rich in fatty acids, with a special focus on eicosapentaenoic acid, an omega-3 fatty acid mainly obtained from marine animal sources, with high importance for human health. N. oceanica has a rigid cell wall constraining protein extraction, thus hydrolyzing it may help increase its components' extractability. Therefore, a Box-Behnken experimental design was carried out to optimize the hydrolysis. The hydrolysate A showed 67±0.7% of protein, antioxidant activity of 1166±63.7 μmol TE/g of protein and an ACE inhibition with an IC50 of 379 μg protein/mL. The hydrolysate B showed 60±1.8% of protein, antioxidant activity of 775±13.0 μmol TE/g of protein and an ACE inhibition with an IC50 of 239 μg protein/mL. The by-product showed higher yields of total fatty acids when compared to “raw” microalgae, being 5.22 and 1%, respectively. The sustainable developed methodology led to the production of one fraction rich in bioactive peptides and another with interesting EPA content, both with value-added properties with potential to be commercialized as ingredients for different industrial applications, such as functional food, supplements or cosmetic formulations.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.doi10.1002/biot.202300291pt_PT
dc.identifier.eid85185340645
dc.identifier.issn1860-6768
dc.identifier.pmid38013664
dc.identifier.urihttp://hdl.handle.net/10400.14/44110
dc.identifier.wos001166757700001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectBioactive peptidespt_PT
dc.subjectEicosapentaenoic acidpt_PT
dc.subjectFunctional foodspt_PT
dc.subjectMicroalgaept_PT
dc.subjectMarine hydrolysatespt_PT
dc.subjectSustainabilitypt_PT
dc.titleEnzymatic hydrolysis allows an integral valorization of nannochloropsis oceanica resulting in the production of bioactive peptide extracts and an eicosapentaenoic acid enriched fractionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue2pt_PT
oaire.citation.titleBiotechnology Journalpt_PT
oaire.citation.volume19pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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