Publication
Enzymatic hydrolysis allows an integral valorization of nannochloropsis oceanica resulting in the production of bioactive peptide extracts and an eicosapentaenoic acid enriched fraction
dc.contributor.author | Cunha, Sara A. | |
dc.contributor.author | Coscueta, Ezequiel R. | |
dc.contributor.author | Alexandre, Agostinho M. R. C. | |
dc.contributor.author | Partidário, Ana Maria Carvalho | |
dc.contributor.author | Fernández, Naiara | |
dc.contributor.author | Paiva, Alexandre | |
dc.contributor.author | Silva, Joana Laranjeira | |
dc.contributor.author | Pintado, Manuela E. | |
dc.date.accessioned | 2024-02-29T11:35:24Z | |
dc.date.available | 2024-11-27T01:31:08Z | |
dc.date.issued | 2024-02 | |
dc.description.abstract | Nannochloropsis oceanica is a microalga with relevant protein content, making it a potential source of bioactive peptides. Furthermore, it is also rich in fatty acids, with a special focus on eicosapentaenoic acid, an omega-3 fatty acid mainly obtained from marine animal sources, with high importance for human health. N. oceanica has a rigid cell wall constraining protein extraction, thus hydrolyzing it may help increase its components' extractability. Therefore, a Box-Behnken experimental design was carried out to optimize the hydrolysis. The hydrolysate A showed 67±0.7% of protein, antioxidant activity of 1166±63.7 μmol TE/g of protein and an ACE inhibition with an IC50 of 379 μg protein/mL. The hydrolysate B showed 60±1.8% of protein, antioxidant activity of 775±13.0 μmol TE/g of protein and an ACE inhibition with an IC50 of 239 μg protein/mL. The by-product showed higher yields of total fatty acids when compared to “raw” microalgae, being 5.22 and 1%, respectively. The sustainable developed methodology led to the production of one fraction rich in bioactive peptides and another with interesting EPA content, both with value-added properties with potential to be commercialized as ingredients for different industrial applications, such as functional food, supplements or cosmetic formulations. | pt_PT |
dc.description.version | info:eu-repo/semantics/acceptedVersion | pt_PT |
dc.identifier.doi | 10.1002/biot.202300291 | pt_PT |
dc.identifier.eid | 85185340645 | |
dc.identifier.issn | 1860-6768 | |
dc.identifier.pmid | 38013664 | |
dc.identifier.uri | http://hdl.handle.net/10400.14/44110 | |
dc.identifier.wos | 001166757700001 | |
dc.language.iso | eng | pt_PT |
dc.peerreviewed | yes | pt_PT |
dc.subject | Bioactive peptides | pt_PT |
dc.subject | Eicosapentaenoic acid | pt_PT |
dc.subject | Functional foods | pt_PT |
dc.subject | Microalgae | pt_PT |
dc.subject | Marine hydrolysates | pt_PT |
dc.subject | Sustainability | pt_PT |
dc.title | Enzymatic hydrolysis allows an integral valorization of nannochloropsis oceanica resulting in the production of bioactive peptide extracts and an eicosapentaenoic acid enriched fraction | pt_PT |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.citation.issue | 2 | pt_PT |
oaire.citation.title | Biotechnology Journal | pt_PT |
oaire.citation.volume | 19 | pt_PT |
rcaap.rights | openAccess | pt_PT |
rcaap.type | article | pt_PT |