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Comparative catalytic activity of two plant proteinases upon caprine caseins in solution

2000Journal article Open access
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dc.contributor.authorSilva, Sofia V.
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2011-10-22T12:40:05Z
dc.date.available2011-10-22T12:40:05Z
dc.date.issued2000
dc.description.abstractThe proteolytic activities of cardosins A and B, two (plant) proteinases from Cynara cardunculus, toward caprine caseins, independently, or in the presence of each other as Na-caseinate, were studied in a comparative fashion using polyacrylamide gel electrophoresis and reversed phase high performance liquid chromatography. The electrophoretic degradation patterns of both αs- and β-casein, brought about by the cardosins, were similar to one another. In what concerns the specificity of these two enzymes upon caseinate, the major cleavage sites were Leu127-Thr128 and Leu190-Tyr191, both in β-casein. When caseins were tested independently, both cardosins cleaved Phe153-Tyr154 in αs1-casein, as well as Leu127-Thr128 and Leu190-Tyr191 in β-casein.por
dc.identifier.citationSILVA Sofia V. ; MALCATA, F. Xavier - Comparative catalytic activity of two plant proteinases upon caprine caseins in solution. Food Chemistry. ISSN 0308-8146. Vol. 71, n.º2 (2000), p. 207-214por
dc.identifier.doi10.1016/S0308-8146(00)00157-6
dc.identifier.urihttp://hdl.handle.net/10400.14/6819
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectGoatpor
dc.subjectRennet substitutepor
dc.subjectProteolysispor
dc.subjectElectrophoresispor
dc.subjectRP-HPLCpor
dc.titleComparative catalytic activity of two plant proteinases upon caprine caseins in solutionpor
dc.typejournal article
dspace.entity.typePublication
person.familyNameMalcata
person.givenNameFrancisco
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0003-3073-1659
person.identifier.scopus-author-id7102542478
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationa06c00da-0e2f-4434-8ede-4d7b22c0dfe9
relation.isAuthorOfPublication.latestForDiscoverya06c00da-0e2f-4434-8ede-4d7b22c0dfe9

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