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A microfluidic paper-based analytical device for the determination of zinc(II) in children’s urine samples and food zinc supplement solutions
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Orientador(es)
Resumo(s)
In this work, an innovative microfluidic paper-based analytical device (?PAD) was developed for the quantification of zinc(II) in biological samples of non-invasive collection, namely children's urine. Zinc(II) in urine can be considered a biomarker of children's psychological development and mental conditions, as well as indicative of urinary tract diseases. The developed device is based on the colourimetric reaction between zinc and dithizone in an alkaline medium and is described as a simple, low-cost method with potential on-site applications. The colour reaction takes about 5 min, and the resulting signal remains stable for 30 min. It enables the quantification of zinc(II) within the dynamic range of 50.0–750 ?g/L, with a limit of detection (LOD) of 10 ?g/L and a limit of quantification (LOQ) of 34 ?g/L. After the device's assembly, it remains stable for use for up to one week when stored at room temperature, either in air or vacuum conditions. The method validation was performed by comparing the results obtained using the ?PAD with those from atomic absorption spectrometry (AAS), where urine samples were analysed directly, or diluted (1:2) when necessary. The developed ?PAD offers a user-friendly, portable, and cost-effective approach for the monitoring of zinc(II) levels in children's urine, providing point-of-care analysis in clinical diagnostics and public health monitoring.
Descrição
Palavras-chave
Children's health monitoring Children’s health monitoring Real-time analysis Urine samples Zinc(II) determination ?PAD
Contexto Educativo
Citação
Melo, M. M. P., Rangel, A. O. S. S., & Mesquita, R. B. R. (in press). A microfluidic paper-based analytical device for the determination of zinc(II) in children’s urine samples and food zinc supplement solutions. Talanta, 298(part A), Article 128865. https://doi.org/10.1016/j.talanta.2025.128865