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Development of a new microfluidic paper-based device (μPAD) for total phenolic content determination of beverages by the fast blue BB method

2026-11-15Artigo de investigação Acesso aberto
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dc.contributor.authorZugazua, Maider
dc.contributor.authorBordagaray, Ane
dc.contributor.authorOstra, Miren
dc.contributor.authorRangel, António O. S. S.
dc.contributor.authorMesquita, Raquel B. R.
dc.date.accessioned2026-09-24T16:01:58Z
dc.date.available2026-09-24T16:01:58Z
dc.date.issued2026-11-15
dc.description.abstractPhenolic compounds constitute a broad family of secondary metabolites synthesized by plants responsible for their color, flavor, and astringency, as well as being very important in the human diet as the main source of antioxidants. This work describes a simple and disposable microfluidic paper-based analytical device (μPAD) for the determination of total phenolic content in different beverages. The detection was based on the colorimetric reaction between the Fast Blue BB reagent and the active hydroxyl groups of the phenolic compounds in an alkaline medium. The developed μPAD enabled TPC determination for gallic acid equivalents in the range of 5–200 mg/L and catechin equivalents of 25–125 mg/L, with a detection limit below 5.5 mg/L. Tea and wine samples were analyzed with the developed μPAD and compared to the conventional spectrophotometric method, with no significant differences (p > 0.05). The device remained stable for 7 days under a vacuum atmosphere.eng
dc.identifier.doi10.1016/j.foodchem.2026.151191
dc.identifier.eid105050765791
dc.identifier.other07cd85df-9036-47e4-b4be-84f71f2e6404
dc.identifier.pmid42753551
dc.identifier.urihttp://hdl.handle.net/10400.14/59577
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Ltd.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMicrofluidic paper-based deviceeng
dc.subjectTotal phenolic contenteng
dc.subjectFast blue BBeng
dc.subjectWineeng
dc.subjectTeaseng
dc.titleDevelopment of a new microfluidic paper-based device (μPAD) for total phenolic content determination of beverages by the fast blue BB method
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.volume529
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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