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Enantiomeric fraction evaluation of pharmaceuticals in environmental matrices by liquid chromatography-tandem mass spectrometry

2014Journal article Restricted access
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dc.contributor.authorRibeiro, Ana Rita
dc.contributor.authorSantos, Lúcia H. M. L. M.
dc.contributor.authorMaia, Alexandra S.
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorCastro, Paula M. L.
dc.contributor.authorTiritan, Maria Elizabeth
dc.date.accessioned2016-01-08T15:03:15Z
dc.date.available2016-01-08T15:03:15Z
dc.date.issued2014
dc.description.abstractThe interest for environmental fate assessment of chiral pharmaceuticals is increasing and enantioselective analytical methods are mandatory. This study presents an enantioselective analytical method for the quantification of seven pairs of enantiomers of pharmaceuticals and a pair of a metabolite. The selected chiral pharmaceuticals belong to three different therapeutic classes, namely selective serotonin reuptake inhibitors (venlafaxine, fluoxetine and its metabolite norfluoxetine), beta-blockers (alprenolol, bisoprolol, metoprolol, propranolol) and a beta2-adrenergic agonist (salbutamol). The analytical method was based on solid phase extraction followed by liquid chromatography tandem mass spectrometry with a triple quadrupole analyser. Briefly, Oasis® MCX cartridges were used to preconcentrate 250 mL of water samples and the reconstituted extracts were analysed with a Chirobiotic™ V under reversed mode. The effluent of a laboratory-scale aerobic granular sludge sequencing batch reactor (AGS-SBR) was used to validate the method. Linearity (r2 > 0.99), selectivity and sensitivity were achieved in the range of 20–400 ng L−1 for all enantiomers, except for norfluoxetine enantiomers which range covered 30–400 ng L−1. The method detection limits were between 0.65 and 11.5 ng L−1 and the method quantification limits were between 1.98 and 19.7 ng L−1. The identity of all enantiomers was confirmed using two MS/MS transitions and its ion ratios, according to European Commission Decision 2002/657/EC. This method was successfully applied to evaluate effluents of wastewater treatment plants (WWTP) in Portugal. Venlafaxine and fluoxetine were quantified as non-racemic mixtures (enantiomeric fraction ≠ 0.5). The enantioselective validated method was able to monitor chiral pharmaceuticals in WWTP effluents and has potential to assess the enantioselective biodegradation in bioreactors. Further application in environmental matrices as surface and estuarine waters can be exploited.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationRibeiro, A. R., Santos, L. H. M. L. M., Maia, A. S., Delerue-Matos, C., Castro, P. M. L., & Tiritan, M. E. (2014). Enantiomeric fraction evaluation of pharmaceuticals in environmental matrices by liquid chromatography-tandem mass spectrometry. Journal of Chromatography A, 1363, 226–235. https://doi.org/10.1016/j.chroma.2014.06.099pt_PT
dc.identifier.doi10.1016/j.chroma.2014.06.099pt_PT
dc.identifier.issn0021-9673
dc.identifier.urihttp://hdl.handle.net/10400.14/18871
dc.identifier.wosWOS:000343359100028
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier
dc.relationFLUOROPHARMA - Biodegradation and removal of chiral and achiral fluorinated pharmaceuticals from wastewaters
dc.relationStrategic Project - UI 4040 - 2011-2012
dc.subjectLC-MS/MSpt_PT
dc.subjectChiral pharmaceuticalspt_PT
dc.subjectEnantiomeric fractionpt_PT
dc.subjectMacrocyclic antibiotic CSPpt_PT
dc.subjectChirobiotic Vpt_PT
dc.titleEnantiomeric fraction evaluation of pharmaceuticals in environmental matrices by liquid chromatography-tandem mass spectrometrypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleFLUOROPHARMA - Biodegradation and removal of chiral and achiral fluorinated pharmaceuticals from wastewaters
oaire.awardTitleStrategic Project - UI 4040 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F64999%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F86939%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEBB-EBI%2F111699%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FSAU%2FUI4040%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PEst-C%2FEQB%2FLA0006%2F2013/PT
oaire.citation.endPage235
oaire.citation.startPage226
oaire.citation.titleJournal of Chromatography A
oaire.citation.volume1363
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCOMPETE
person.familyNameRibeiro
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person.familyNameMaia
person.familyNameDelerue-Matos
person.familyNameCastro
person.familyNameTiritan
person.givenNameAna Rita Lado
person.givenNameLúcia
person.givenNameAlexandra S.
person.givenNameCristina
person.givenNamePaula
person.givenNameMaria Elizabeth
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person.identifier.scopus-author-id57207918943
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project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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