Publication
Image analysis semi-automatic system for colony-forming-unit counting
| dc.contributor.author | Rodrigues, Pedro Miguel | |
| dc.contributor.author | Luís, Jorge | |
| dc.contributor.author | Tavaria, Freni Kekhasharú | |
| dc.date.accessioned | 2022-07-19T09:58:49Z | |
| dc.date.available | 2022-07-19T09:58:49Z | |
| dc.date.issued | 2022-07 | |
| dc.description.abstract | Background: Accurate quantitative analysis of microorganisms is recognized as an essential tool for gauging safety and quality in microbiology settings in a wide range of fields. The enumeration process of viable microorganisms via traditional culturing techniques are methodically convenient and cost-effective, conferring high applicability worldwide. However, manual counting can be time-consuming, laborious and imprecise. Furthermore, particular cases require an urgent and accurate response for effective processing. Methods: To reduce time limitations and discrepancies, this work introduces an image processing method capable of semi-automatically quantifying the number of colony forming units (CFUs). This rapid enumeration technique enables the technician to provide an expeditious assessment of the microbial load of a given sample. To test and validate the system, three bacterial species were cultured, and a labeled database was created, with subsequent image acquisition. Results: The system demonstrated acceptable classification measures; the mean values of Accuracy, Recall and F-measure were: (1) 95%, 95% and 0.95 for E. coli; (2) 91%, 91% and 0.90 for P. aeruginosa; and (3) 84%, 86% and 0.85 for S. aureus. Conclusions: Evidence related to the time-saving potential of the system was achieved; the time spent on quantification tasks of plates with a high number of colonies might be reduced to a half and occasionally to a third. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.3390/bioengineering9070271 | pt_PT |
| dc.identifier.eid | 85133201772 | |
| dc.identifier.issn | 2306-5354 | |
| dc.identifier.pmc | PMC9312004 | |
| dc.identifier.pmid | 35877322 | |
| dc.identifier.uri | http://hdl.handle.net/10400.14/38274 | |
| dc.identifier.wos | 000833244600001 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Colony forming units | pt_PT |
| dc.subject | Enumeration | pt_PT |
| dc.subject | Image processing | pt_PT |
| dc.subject | Petri-plates | pt_PT |
| dc.title | Image analysis semi-automatic system for colony-forming-unit counting | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 7 | pt_PT |
| oaire.citation.title | Bioengineering | pt_PT |
| oaire.citation.volume | 9 | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |