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High throughput analysis of integron gene cassettes in wastewater environments

2016Journal article Restricted access
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dc.contributor.authorGatica, Joao
dc.contributor.authorTripathi, Vijay
dc.contributor.authorGreen, Stefan
dc.contributor.authorManaia, Célia M.
dc.contributor.authorBerendonk, Thomas
dc.contributor.authorCacace, Damiano
dc.contributor.authorMerlin, Christophe
dc.contributor.authorKreuzinger, Norbert
dc.contributor.authorSchwartz, Thomas
dc.contributor.authorFatta-Kassinos, Despo
dc.contributor.authorRizzo, Luigi
dc.contributor.authorSchwermer, Carsten U.
dc.contributor.authorGarelick, Hemda
dc.contributor.authorJurkevitch, Edouard
dc.contributor.authorCytryn, Eddie
dc.date.accessioned2017-10-11T14:58:15Z
dc.date.available2017-10-11T14:58:15Z
dc.date.issued2016
dc.description.abstractIntegrons are extensively targeted as a proxy for anthropogenic impact in the environment. We developed a novel high-throughput amplicon sequencing pipeline that enables characterization of thousands of integron gene cassette-associated reads, and applied it to acquire a comprehensive overview of gene cassette composition in effluents from wastewater treatment facilities across Europe. Between 38 100 and 172 995 reads per-sample were generated and functionally characterized by screening against nr, SEED, ARDB and β-lactamase databases. Over 75% of the reads were characterized as hypothetical, but thousands were associated with toxin-antitoxin systems, DNA repair, cell membrane function, detoxification and aminoglycoside and β-lactam resistance. Among the reads characterized as β-lactamases, the carbapenemase blaOXA was dominant in most of the effluents, except for Cyprus and Israel where blaGES was also abundant. Quantitative PCR assessment of blaOXA and blaGES genes in the European effluents revealed similar trends to those displayed in the integron amplicon sequencing pipeline described above, corroborating the robustness of this method and suggesting that these integron-associated genes may be excellent targets for source tracking of effluents in downstream environments. Further application of the above analyses revealed several order-of-magnitude reductions in effluent-associated β-lactamase genes in effluent-saturated soils, suggesting marginal persistence in the soil microbiome.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGATICA, Joao; TRIPATHI, Vijay; GREEN, Stefan; MANAIA, Celia M.; BERENDONK, Thomas; CACACE, Damiano; MERLIN, Christophe; KREUZINGER, Norbert; SCHWARTZ, Thomas; FATTA-KASSINOS, Despo; RIZZO, Luigi; SCHWERMER, Carsten U.; GARELICK, Hemda; JURKEVITCH, Edouard; CYTRYN, Eddie - High Throughput Analysis of Integron Gene Cassettes in Wastewater Environments. Environmental Science & Technology. ISSN 1520-5851. Vol. 50, n.º 21 (2016), p. 11825-11836pt_PT
dc.identifier.doi10.1021/acs.est.6b03188pt_PT
dc.identifier.eid84993927168
dc.identifier.pmid27689892
dc.identifier.urihttp://hdl.handle.net/10400.14/22965
dc.identifier.wos000386991100045
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Chemical Society
dc.titleHigh throughput analysis of integron gene cassettes in wastewater environmentspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage11836
oaire.citation.issue21
oaire.citation.startPage11825
oaire.citation.titleEnvironmental Science and Technologypt_PT
oaire.citation.volume50
person.familyNameTripathi
person.familyNameGreen
person.familyNameManaia
person.familyNameCacace
person.familyNameMerlin
person.familyNameKreuzinger
person.familyNameFatta-Kassinos
person.familyNameRizzo
person.familyNameSchwermer
person.givenNameVijay
person.givenNameStefan
person.givenNameCélia
person.givenNameDamiano
person.givenNameChristophe
person.givenNameNorbert
person.givenNameDespo
person.givenNameLuigi
person.givenNameCarsten Ulrich
person.identifier44816
person.identifier.ciencia-idC31F-553B-6365
person.identifier.orcid0000-0002-6103-0890
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person.identifier.orcid0000-0002-9261-5322
person.identifier.orcid0000-0002-6400-8218
person.identifier.orcid0000-0003-1173-0941
person.identifier.orcid0000-0003-1449-9116
person.identifier.orcid0000-0002-6987-4879
person.identifier.ridG-2331-2016
person.identifier.ridA-6286-2008
person.identifier.ridB-7442-2014
person.identifier.scopus-author-id55229529300
person.identifier.scopus-author-id7403569147
person.identifier.scopus-author-id6602465318
person.identifier.scopus-author-id6602188649
person.identifier.scopus-author-id6603513157
person.identifier.scopus-author-id6603838891
person.identifier.scopus-author-id9044416100
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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