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Bacterial lineages putatively associated with the dissemination of antibiotic resistance genes in a full-scale urban wastewater treatment plant

2018Journal article Open access
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dc.contributor.authorNarciso-da-Rocha, Carlos
dc.contributor.authorRocha, Jaqueline
dc.contributor.authorVaz-Moreira, Ivone
dc.contributor.authorLira, Felipe
dc.contributor.authorTamames, Javier
dc.contributor.authorHenriques, Isabel
dc.contributor.authorMartinez, Jose Luis
dc.contributor.authorManaia, Célia M.
dc.date.accessioned2018-06-04T14:02:45Z
dc.date.available2018-06-04T14:02:45Z
dc.date.issued2018
dc.description.abstractUrban wastewater treatment plants (UWTPs) are reservoirs of antibiotic resistance. Wastewater treatment changes the bacterial community and inevitably impacts the fate of antibiotic resistant bacteria and antibiotic resistance genes (ARGs). Some bacterial groups are major carriers of ARGs and hence, their elimination during wastewater treatment may contribute to increasing resistance removal efficiency. This study, conducted at a full-scale UWTP, evaluated variations in the bacterial community and ARGs loads and explored possible associations among them. With that aim, the bacterial community composition (16S rRNA gene Illumina sequencing) and ARGs abundance (real-time PCR) were characterized in samples of raw wastewater (RWW), secondary effluent (sTWW), after UV disinfection (tTWW), and after a period of 3 days storage to monitoring possible bacterial regrowth (tTWW-RE). Culturable enterobacteria were also enumerated. Secondary treatment was associated with the most dramatic bacterial community variations and coincided with reductions of ~2 log-units in the ARGs abundance. In contrast, no significant changes in the bacterial community composition and ARGs abundance were observed after UV disinfection of sTWW. Nevertheless, after UV treatment, viability losses were indicated ~2 log-units reductions of culturable enterobacteria. The analysed ARGs (qnrS, blaCTX-M, blaOXA-A, blaTEM, blaSHV, sul1, sul2, and intI1) were strongly correlated with taxa more abundant in RWW than in the other types of water, and which associated with humans and animals, such as members of the families Campylobacteraceae, Comamonadaceae, Aeromonadaceae, Moraxellaceae and Bacteroidaceae. Further knowledge of the dynamics of the bacterial community during wastewater treatment and its relationship with ARGs variations may contribute with information useful for wastewater treatment optimization, aiming at a more effective resistance control.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationNarciso-da-Rocha, C., Rocha, J., Vaz-Moreira, I., Lira, F., Tamames, J., Henriques, I… Manaia, C. M. (2018). Bacterial lineages putatively associated with the dissemination of antibiotic resistance genes in a full-scale urban wastewater treatment plant. Environment International. 54 p.pt_PT
dc.identifier.doi10.1016/j.envint.2018.05.040
dc.identifier.eid85047923292
dc.identifier.eissn1873-6750
dc.identifier.issn0160-4120
dc.identifier.pmid29883764
dc.identifier.urihttp://hdl.handle.net/10400.14/24536
dc.identifier.wos000438183000021
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationNORTE-08-5369-FSE-000007pt_PT
dc.relationCTM2013-48292-C3-2-Rpt_PT
dc.relationNEW INSIGHTS INTO THE ENVIRONMENTAL ANTIBIOTIC RESISTOME
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectWastewater treatmentpt_PT
dc.subjectBacterial community dynamicspt_PT
dc.subjectAntibiotic resistance genespt_PT
dc.subjectNetwork analysispt_PT
dc.titleBacterial lineages putatively associated with the dissemination of antibiotic resistance genes in a full-scale urban wastewater treatment plantpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNEW INSIGHTS INTO THE ENVIRONMENTAL ANTIBIOTIC RESISTOME
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/WaterJPI%2F0001%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAMB%2F50017%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F87360%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F97131%2F2013/PT
oaire.citation.endPage188
oaire.citation.startPage179
oaire.citation.titleEnvironment Internationalpt_PT
oaire.citation.volume118
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNameNarciso-da-Rocha
person.familyNameRocha
person.familyNameVaz-Moreira
person.familyNameTamames
person.familyNameHenriques
person.familyNameMartinez
person.familyNameManaia
person.givenNameCarlos
person.givenNameJaqueline
person.givenNameIvone
person.givenNameJavier
person.givenNameIsabel
person.givenNameJose Luis
person.givenNameCélia
person.identifier602434
person.identifier19844
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person.identifier.ciencia-idC31F-553B-6365
person.identifier.orcid0000-0001-6398-1633
person.identifier.orcid0000-0003-1651-9483
person.identifier.orcid0000-0001-8912-9836
person.identifier.orcid0000-0003-4547-8932
person.identifier.orcid0000-0001-7717-4939
person.identifier.orcid0000-0001-8813-7607
person.identifier.orcid0000-0002-3674-1789
person.identifier.ridK-3850-2014
person.identifier.ridA-4725-2013
person.identifier.scopus-author-id55556396600
person.identifier.scopus-author-id57113035100
person.identifier.scopus-author-id16044425800
person.identifier.scopus-author-id6602002725
person.identifier.scopus-author-id6701586581
person.identifier.scopus-author-id6602465318
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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