Logo do repositório
 
Publicação

Human umbilical cord blood plasma as an alternative to animal sera for mesenchymal stromal cells in vitro expansion – A multicomponent metabolomic analysis

2018Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorCaseiro, A. R.
dc.contributor.authorIvanova, G.
dc.contributor.authorPedrosa, S. S.
dc.contributor.authorBranquinho, M. V.
dc.contributor.authorGeorgieva, P.
dc.contributor.authorBarbosa, P. P.
dc.contributor.authorMauricio, A. C.
dc.contributor.authorTeixeira, P.
dc.date.accessioned2018-11-16T18:52:42Z
dc.date.available2018-11-16T18:52:42Z
dc.date.issued2018
dc.description.abstractMesenchymal Stromal cells (MSCs) have a potential role in cell-based therapies. Foetal bovine serum (FBS) is used to supplement the basal cell culture medium but presents several disadvantages and risks. Other alternatives have been studied, including human umbilical cord blood plasma (hUCBP), aiming at the development of xeno-free culturing protocols. A comparative characterization of multicomponent metabolic composition of hUCBP and commercial FBS based on Nuclear Magnetic Resonance (NMR) spectroscopy and multivariate statistical analysis was performed. The analysis of 1H-NMR spectra revealed both similarities and differences between the two proposed supplements. Similar metabolites (amino acids, glucose, lipids and nucleotides) were found in the hUCBP and FBS NMR spectra. The results show that the major difference between the metabolic profiles of the two proposed supplements are due to the significantly higher levels of glucose and lower levels of lactate, glutamate, alanine and branched chain amino acids in hUCBP. Similar or slightly different levels of important proteinogenic amino acids, as well as of nucleotides, lipids were found in the hUCBP and FBS. In order to validate it’s suitability for cell culture, umbilical cord-MSCs (UC-MSCs) and dental pulp stem cells (DPSCs) were expanded using hUCBP. In both hMSCs, in vitro culture with hUCBP supplementation presented similar to improved metabolic performances when compared to FBS. The two cell types tested expressed different optimum hUCBP percentage content. For DPSCs, the optimum hUCBP content was 6% and for UC-MSCs, 4%. Cultured hMSCs displayed no changes in senescence indicators, as well as maintained characteristic surface marker’s expression. FBS substitution was associated with an increase in early apoptosis events, in a dose dependent manner, as well as to slight up- and down-regulation of targeted gene’s expression. Tri-lineage differentiation capacity was also influenced by the substitution of FBS by hUCBP.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCaseiro, A.R., Ivanova, G., Pedrosa, S.S., Branquinho, M.V., Georgieva, P., Barbosa, P.P., …Maurício, C. (2018). Human umbilical cord blood plasma as an alternative to animal sera for mesenchymal stromal cells in vitro expansion – A multicomponent metabolomic analysis. PLoS ONE, 13(10), art. n.º e0203936pt_PT
dc.identifier.doi10.1371/journal.pone.0203936pt_PT
dc.identifier.eid85054706834
dc.identifier.issn1932-6203
dc.identifier.pmcPMC6179201
dc.identifier.pmid30304014
dc.identifier.urihttp://hdl.handle.net/10400.14/26047
dc.identifier.wos000446921100022
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPublic Library Science
dc.relationNORTE-01-0247-FEDER-003262
dc.relationStrategic Project - UI 211 - 2011-2012
dc.relationStrategic Project - UI 285 - 2013-2014
dc.relationBioMaTE - A novel bio-manufacturing system to produce bioactive scaffolds for tissue engineering
dc.relationSkeletal muscle regeneration through the application of cellular therapies and biomaterials - Translation to the veterinary practice alterado para: “Cell-based Therapies in Regenerative Medicine – The therapeuthic potential of Umbilical Cord and Dental Pulp derived Stem / Stromal Cells”
dc.relationPOCI-01-0247-FEDER-017771
dc.relationNORTE-07-0124-FEDER-000066
dc.relationNORTE-07-0162-FEDER-000048
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleHuman umbilical cord blood plasma as an alternative to animal sera for mesenchymal stromal cells in vitro expansion – A multicomponent metabolomic analysispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 211 - 2011-2012
oaire.awardTitleStrategic Project - UI 285 - 2013-2014
oaire.awardTitleBioMaTE - A novel bio-manufacturing system to produce bioactive scaffolds for tissue engineering
oaire.awardTitleSkeletal muscle regeneration through the application of cellular therapies and biomaterials - Translation to the veterinary practice alterado para: “Cell-based Therapies in Regenerative Medicine – The therapeuthic potential of Umbilical Cord and Dental Pulp derived Stem / Stromal Cells”
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FAGR%2FUI0211%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FEME%2FUI0285%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FEMS-SIS%2F7032%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F101174%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.citation.issue10
oaire.citation.titlePLoS ONEpt_PT
oaire.citation.volume13
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6820 - DCRRNI ID
oaire.fundingStream9471 - RIDTI
oaire.fundingStreamFARH
oaire.fundingStream5876
person.familyNameCaseiro Santos
person.familyNameIvanova
person.familyNameAlmeida Santos Pedrosa
person.familyNameVieira Branquinho
person.familyNameGeorgieva
person.familyNameMaurício
person.familyNameTeixeira
person.givenNameAna Rita
person.givenNameGalya
person.givenNameSílvia Marlene
person.givenNameMariana Esteves
person.givenNamePetia
person.givenNameAna Colette
person.givenNamePaula
person.identifierR-00H-YD9
person.identifierR-000-F4E
person.identifier.ciencia-id6F17-F69C-59F6
person.identifier.ciencia-id4913-3E4B-CB57
person.identifier.ciencia-idE51E-FD73-9E69
person.identifier.ciencia-id721E-6029-C666
person.identifier.ciencia-id6D1E-22DA-1F69
person.identifier.ciencia-id901B-F8EB-BD8D
person.identifier.orcid0000-0002-1143-2461
person.identifier.orcid0000-0003-3195-634X
person.identifier.orcid0000-0002-2055-8031
person.identifier.orcid0000-0003-1328-464X
person.identifier.orcid0000-0002-6424-6590
person.identifier.orcid0000-0002-0018-9363
person.identifier.orcid0000-0002-6296-5137
person.identifier.ridD-1845-2018
person.identifier.ridG-5805-2011
person.identifier.ridP-7255-2016
person.identifier.ridE-8917-2013
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id56287438400
person.identifier.scopus-author-id7102078113
person.identifier.scopus-author-id57203143007
person.identifier.scopus-author-id6701326860
person.identifier.scopus-author-id12143945000
person.identifier.scopus-author-id7005895206
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationcd5560e3-90f3-4c65-9c51-818e3b3c1997
relation.isAuthorOfPublicatione0d2c6ce-5105-44c2-bb2b-d531858fb5e4
relation.isAuthorOfPublicationd5603761-15b0-4cd3-8aa2-7fd4bb1bc836
relation.isAuthorOfPublicationfd475826-624c-4661-954b-d151190d74f2
relation.isAuthorOfPublicationd19151be-eef8-497e-9b4f-2ca6889c2732
relation.isAuthorOfPublicationc7b82486-6287-41a8-a74a-274ca660626b
relation.isAuthorOfPublicationb918d4a2-b9f9-4c56-8318-111625e4d58b
relation.isAuthorOfPublication.latestForDiscoveryfd475826-624c-4661-954b-d151190d74f2
relation.isProjectOfPublication89cfc861-31bf-4da7-9170-514f29ef0762
relation.isProjectOfPublicationdc8e4473-87cc-455e-9835-df38569d5eee
relation.isProjectOfPublicationa65b7d18-db0f-4471-a371-ce542310c15f
relation.isProjectOfPublication0be81491-594d-4cc6-8a14-f55907eb9731
relation.isProjectOfPublication3fe5970e-de39-42f7-a29d-18521b591a09
relation.isProjectOfPublication.latestForDiscoverya65b7d18-db0f-4471-a371-ce542310c15f

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
19778227.pdf
Tamanho:
6.19 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
3.44 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

CBQF - Contribuições em Revistas Científicas / Contribution to Journals