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Exploring silk Sericin for diabetic wounds: an in situ-forming hydrogel to protect against oxidative stress and improve tissue healing and regeneration

2022-06-08Journal article Open access
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dc.contributor.authorBaptista-Silva, Sara
dc.contributor.authorBernardes, Beatriz G.
dc.contributor.authorBorges, Sandra
dc.contributor.authorRodrigues, Ilda
dc.contributor.authorFernandes, Rui
dc.contributor.authorGomes-Guerreiro, Susana
dc.contributor.authorPinto, Marta Teixeira
dc.contributor.authorPintado, Manuela
dc.contributor.authorSoares, Raquel
dc.contributor.authorCosta, Raquel
dc.contributor.authorOliveira, Ana Leite
dc.date.accessioned2022-07-22T16:35:42Z
dc.date.available2022-07-22T16:35:42Z
dc.date.issued2022-06-08
dc.description.abstractChronic wounds are one of the most frequent complications that are associated with diabetes mellitus. The overproduction of reactive oxygen species (ROS) is a key factor in the delayed healing of a chronic wound. In the present work, we develop a novel in situ-forming silk sericin-based hydrogel (SSH) that is produced by a simple methodology using horseradish peroxidase (HRP) crosslinking as an advanced dressing for wound healing. The antioxidant and angiogenic effects were assessed in vitro and in vivo after in situ application using an excisional wound-healing model in a genetically-induced diabetic db/db mice and though the chick embryo choriollantoic membrane (CAM) assay, respectively. Wounds in diabetic db/db mice that were treated with SSH closed with reduced granulation tissue, decreased wound edge distance, and wound thickness, when compared to Tegaderm, a dressing that is commonly used in the clinic. The hydrogel also promoted a deposition of collagen fibers with smaller diameter which may have had a boost effect in re-epithelialization. SSH treatment slightly induced two important endogenous antioxidant defenses, superoxide dismutase and catalase. A CAM assay made it possible to observe that SSH led to an increase in the number of newly formed vessels without inducing an inflammatory reaction. The present hydrogel may result in a multi-purpose technology with angiogenic, antioxidant, and anti-inflammatory properties, while advancing efficient and organized tissue regeneration.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/biom12060801pt_PT
dc.identifier.eid85131410480
dc.identifier.issn2218-273X
dc.identifier.pmcPMC9221298
dc.identifier.pmid35740928
dc.identifier.urihttp://hdl.handle.net/10400.14/38363
dc.identifier.wos000916417100001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHorseradish peroxidasept_PT
dc.subjectHydrogelpt_PT
dc.subjectIn situ formingpt_PT
dc.subjectSericinpt_PT
dc.subjectSilkpt_PT
dc.subjectWound healingpt_PT
dc.titleExploring silk Sericin for diabetic wounds: an in situ-forming hydrogel to protect against oxidative stress and improve tissue healing and regenerationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue6pt_PT
oaire.citation.titleBiomoleculespt_PT
oaire.citation.volume12pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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