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Advisor(s)
Abstract(s)
Aim Plant -associated bacteria can improve
phytoextraction by increasing plant growth and/or metal
uptake. This study aimed to characterise the culturable
rhizobacterial community associated with two Nihyperaccumulators
and to obtain a collection of isolates
for application in Ni phytomining.
Methods Non-vegetated and rhizosphere soil samples
were collected from the Ni-hyperaccumulator Alyssum
serpyllifolium ssp. lusitanicum (three populations) and
Alyssum serpyllifolium ssp. malacitanum (one population),
as well as from non-hyperaccumulating plants
(Dactylis glomerata, Santolina semidentata and
Alyssum serpyllifolium ssp. serpyllifolium).
Rhizobacteria were isolated and characterised genotypically
(BOX-PCR, 16S rDNA sequencing) and phenotypically
(Ni tolerance, plant growth promoting (PGP)
traits, biosurfactant production).
Results Hyperaccumulating Alyssum subspecies hosted
higher densities of bacteria compared to either nonhyperaccumulators
or non-vegetated soil. In some cases
hyperaccumulators showed selective enrichment of Nitolerant
bacteria. Most bacterial strains belonged to the
Actinobacteria phylum and presented Ni resistance.
Phosphorus-solubilisers were mostly associated with
the hyperaccumulators, siderophore-producers with
D. glomerata, and IAA-producers with both these
species.
Conclusion Taxonomic diversity and phenotypic characteristics
were soil-, plant species- and plant population-
specific. Moreover, differences were observed between
the two Ni-hyperaccumulating subspecies and
amongst plant populations. Several strains presented
PGP characteristics which could be useful when
selecting microorganisms for bioaugmentation trials.
Description
Keywords
Alyssum serpyllifolium Hyperaccumulators Nickel Plant-associated bacteria PGP traits Bacterial diversity Phytoextraction
Pedagogical Context
Citation
ÁLVAREZ-LÓPEZ, Vanessa; PRIETO-FERNÁNDEZ, Ángeles; BECERRA-CASTRO, Cristina; MONTERROSO, Carmela; KIDD, Petra S. - Rhizobacterial communities associated with the flora of three serpentine outcrops of the Iberian Peninsula. Plant and Soil. ISSN 0032-079X (2016), vol. 403, nº1-2, p.233-252. DOI 10.1007/s11104-015-2632-0
Publisher
Springer