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Multidrug resistance in quinolone-resistant gram-negative bacteria isolated from hospital effluent and the municipal wastewater treatment plant

2016Journal article Restricted access
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dc.contributor.authorVaz-Moreira, Ivone
dc.contributor.authorVarela, Ana Rita
dc.contributor.authorPereira, Thamiris V.
dc.contributor.authorFochat, Romário C.
dc.contributor.authorManaia, C. M.
dc.date.accessioned2016-04-21T16:59:39Z
dc.date.available2016-04-21T16:59:39Z
dc.date.issued2016
dc.description.abstractThis study is aimed to assess if hospital effluents represent an important supplier of multidrug-resistant (MDR) Gram-negative bacteria that, being discharged in the municipal collector, may be disseminated in the environment and bypassed in water quality control systems. From a set of 101 non-Escherichia coli Gram-negative bacteria with reduced susceptibility to quinolones, was selected a group of isolates comprised by those with the highest indices of MDR (defined as nonsusceptibility to at least one agent in six or more antimicrobial categories, MDR ‡6) or resistance to meropenem or ceftazidime (n = 25). The isolates were identified and characterized for antibiotic resistance phenotype, plasmid-mediated quinolone resistance (PMQR) genes, and other genetic elements and conjugative capacity. The isolates with highest MDR indices were mainly from hospital effluent and comprised ubiquitous bacterial groups of the class Gammaproteobacteria, of the genera Aeromonas, Acinetobacter, Citrobacter, Enterobacter, Klebsiella, and Pseudomonas, and of the class Flavobacteriia, of the genera Chryseobacterium and Myroides. In this group of 25 strains, 19 identified as Gammaproteobacteria harbored at least one PMQR gene (aac(6¢)-Ib-cr, qnrB, qnrS, or oqxAB) or a class 1 integron gene cassette encoding aminoglycoside, sulfonamide, or carbapenem resistance. Most of the E. coli J53 transconjugants with acquired antibiotic resistance resulted from conjugation with Enterobacteriaceae. These transconjugants demonstrated acquired resistance to a maximum of five classes of antibiotics, one or more PMQR genes and/or a class 1 integron gene cassette. This study shows that ubiquitous bacteria, other than those monitored in water quality controls, are important vectors of antibiotic resistance and can be disseminated from hospital effluent to aquatic environments. This information is relevant to support management options aiming at the control of this public health problem.pt_PT
dc.identifier.citationVAZ-MOREIRA, Ivone; Varela, Ana Rita; Pereira, Thamiris V.; Fochat, Romário C.; Manaia, C. M. - Multidrug Resistance in Quinolone-Resistant Gram-Negative Bacteria Isolated from Hospital Effluent and the Municipal Wastewater Treatment Plant. Microbial Drug Resistance, 22(2):155-163. ISSN 1931-8448. (2016), 9 p.pt_PT
dc.identifier.doi10.1089/mdr.2015.0118
dc.identifier.eid84960912246
dc.identifier.issn1076-6294
dc.identifier.pmid26469134
dc.identifier.urihttp://hdl.handle.net/10400.14/19952
dc.identifier.wos000371872100010
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMary Ann Liebert
dc.relationDYNAMICS OF ANTIMICROBIAL RESISTANCE BETWEEN THE HOSPITAL AND THE ENVIRONMENT
dc.relationTRACKING ANTIBIOTIC RESISTANCE FROM HOSPITAL EFFLUENTS TO THE SURROUNDING ENVIRONMENT
dc.titleMultidrug resistance in quinolone-resistant gram-negative bacteria isolated from hospital effluent and the municipal wastewater treatment plantpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDYNAMICS OF ANTIMICROBIAL RESISTANCE BETWEEN THE HOSPITAL AND THE ENVIRONMENT
oaire.awardTitleTRACKING ANTIBIOTIC RESISTANCE FROM HOSPITAL EFFLUENTS TO THE SURROUNDING ENVIRONMENT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAAC-AMB%2F113840%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F70986%2F2010/PT
oaire.citation.endPage163
oaire.citation.issue2
oaire.citation.startPage155
oaire.citation.titleMicrobial Drug Resistance
oaire.citation.volume22
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
person.familyNameVaz-Moreira
person.familyNameVarela
person.familyNameManaia
person.givenNameIvone
person.givenNameAna Rita
person.givenNameCélia
person.identifier602434
person.identifier.ciencia-id9E17-544E-800D
person.identifier.ciencia-idC31F-553B-6365
person.identifier.orcid0000-0001-8912-9836
person.identifier.orcid0000-0001-9691-0217
person.identifier.orcid0000-0002-3674-1789
person.identifier.scopus-author-id16044425800
person.identifier.scopus-author-id6602465318
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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