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Enzymatic determination of L(+) lactic and L(-) malic acids in wines by flow-injection spectrophotometry

dc.contributor.authorLima, José L. F. C.
dc.contributor.authorLopes, Teresa I. M. S.
dc.contributor.authorRangel, António O. S. S.
dc.date.accessioned2011-10-20T19:16:34Z
dc.date.available2011-10-20T19:16:34Z
dc.date.issued1998
dc.description.abstractA flow-injection system for the enzymatic determination of L( ) lactic acid and L(ÿ) malic acid in wines with spectrophotometric detection is described. The samples are dialysed in-line, and the enzymes in solution (malate dehydrogenase and lactate dehydrogenase) are injected as a train of plugs in the acceptor stream of the dialysis unit, yielding two peaks corresponding to the NADH formed for each determination. This methodology enables the determination of both acids with a single detector with a sampling rate of 20 h ÿ1 (0.4±3 g l ÿ1). The results are comparable to those obtained by the reference procedure, the repeatability is better than 5% (rsd), with low enzyme consumption (1.3 ml of suspension per sample).por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationLIMA, José L. F. C.; LOPES, Teresa I. M. S. ; RANGEL António O.S.S. - Enzymatic determination of L(+) lactic and L(-) malic acids in wines by flow-injection spectrophotometry. Analytica Chimica Acta. ISSN 0003-2670. Vol. 366 (1998), p. 187-191por
dc.identifier.doi10.1016/S0003-2670(98)00106-8
dc.identifier.urihttp://hdl.handle.net/10400.14/6580
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectSequential Flow injectionpor
dc.subjectL(+) lactic acidpor
dc.subjectL(-) malic acidpor
dc.subjectSpectrophotometric enzymatic determinationpor
dc.subjectWinespor
dc.titleEnzymatic determination of L(+) lactic and L(-) malic acids in wines by flow-injection spectrophotometrypor
dc.typejournal article
dspace.entity.typePublication
person.familyNameLima
person.familyNameSantos Silva Rangel
person.givenNameJosé
person.givenNameAntónio Osmaro
person.identifierR-000-8FJ
person.identifier.ciencia-id4811-9AA9-D673
person.identifier.ciencia-idD61D-7CFB-178A
person.identifier.orcid0000-0002-8906-9826
person.identifier.orcid0000-0002-6486-8947
person.identifier.scopus-author-id10141079100
person.identifier.scopus-author-id7004827387
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication71f20aa3-d4ae-40c3-a9ef-27d88442d1e0
relation.isAuthorOfPublication467be022-fd9c-4549-aa48-48ebcc1098eb
relation.isAuthorOfPublication.latestForDiscovery467be022-fd9c-4549-aa48-48ebcc1098eb

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